Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   S100333_RS13000 Genome accession   NZ_CP021892
Coordinates   2423209..2424279 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis subsp. subtilis strain SRCM100333     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418209..2429279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S100333_RS12955 (S100333_02650) tapA 2418688..2419449 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  S100333_RS12960 (S100333_02651) yqzG 2419721..2420047 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  S100333_RS12965 (S100333_02652) spoIITA 2420089..2420268 (-) 180 WP_014480252.1 YqzE family protein -
  S100333_RS12970 (S100333_02653) comGG 2420339..2420713 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  S100333_RS12975 (S100333_02654) comGF 2420714..2421097 (-) 384 WP_014480254.1 ComG operon protein ComGF Machinery gene
  S100333_RS12980 (S100333_02655) comGE 2421123..2421470 (-) 348 WP_088272394.1 ComG operon protein 5 Machinery gene
  S100333_RS12985 (S100333_02656) comGD 2421454..2421885 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  S100333_RS12990 (S100333_02657) comGC 2421875..2422171 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  S100333_RS12995 (S100333_02658) comGB 2422185..2423222 (-) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  S100333_RS13000 (S100333_02659) comGA 2423209..2424279 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  S100333_RS13005 (S100333_02660) - 2424491..2424688 (-) 198 WP_014480259.1 CBS domain-containing protein -
  S100333_RS13010 (S100333_02661) corA 2424690..2425643 (-) 954 WP_014480260.1 magnesium transporter CorA -
  S100333_RS13015 (S100333_02662) yqhB 2425785..2427113 (+) 1329 WP_014480261.1 hemolysin family protein -
  S100333_RS13020 (S100333_02663) rsbRD 2427166..2428002 (-) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -
  S100333_RS13030 (S100333_02665) mgsR 2428226..2428606 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  S100333_RS13035 (S100333_02666) yqgY 2428838..2429083 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=234541 S100333_RS13000 WP_004399124.1 2423209..2424279(-) (comGA) [Bacillus subtilis subsp. subtilis strain SRCM100333]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=234541 S100333_RS13000 WP_004399124.1 2423209..2424279(-) (comGA) [Bacillus subtilis subsp. subtilis strain SRCM100333]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment