Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C6P70_RS15130 Genome accession   NZ_CP027388
Coordinates   2763844..2764284 (+) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain 2011C-4251     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2758844..2769284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P70_RS15105 aroP 2759842..2761215 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  C6P70_RS15110 ampE 2761258..2762112 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  C6P70_RS15115 ampD 2762109..2762660 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C6P70_RS15120 nadC 2762748..2763641 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C6P70_RS15130 pilA 2763844..2764284 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  C6P70_RS15135 pilB 2764294..2765679 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  C6P70_RS15140 hofC 2765669..2766871 (+) 1203 WP_000157234.1 protein transport protein HofC -
  C6P70_RS15145 guaC 2766906..2767949 (-) 1044 WP_001217338.1 GMP reductase -
  C6P70_RS29945 - 2768105..2768149 (-) 45 WP_120795372.1 protein YacM -
  C6P70_RS15150 coaE 2768174..2768794 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=233404 C6P70_RS15130 WP_000360894.1 2763844..2764284(+) (pilA) [Escherichia coli strain 2011C-4251]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=233404 C6P70_RS15130 WP_000360894.1 2763844..2764284(+) (pilA) [Escherichia coli strain 2011C-4251]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377

Multiple sequence alignment