Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AYN27_RS22725 Genome accession   NZ_CP027380
Coordinates   4162540..4162980 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain 2013C-3250     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4157540..4167980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYN27_RS22700 aroP 4158538..4159911 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  AYN27_RS22705 ampE 4159954..4160808 (-) 855 WP_000172003.1 beta-lactamase regulator AmpE -
  AYN27_RS22710 ampD 4160805..4161356 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AYN27_RS22715 nadC 4161444..4162337 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AYN27_RS22725 pilA 4162540..4162980 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  AYN27_RS22730 pilB 4162990..4164375 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  AYN27_RS22735 hofC 4164365..4165567 (+) 1203 WP_000157234.1 protein transport protein HofC -
  AYN27_RS22740 guaC 4165602..4166645 (-) 1044 WP_001217338.1 GMP reductase -
  AYN27_RS31425 - 4166801..4166845 (-) 45 WP_120795372.1 protein YacM -
  AYN27_RS22745 coaE 4166870..4167490 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=233268 AYN27_RS22725 WP_000360902.1 4162540..4162980(+) (pilA) [Escherichia coli strain 2013C-3250]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=233268 AYN27_RS22725 WP_000360902.1 4162540..4162980(+) (pilA) [Escherichia coli strain 2013C-3250]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377

Multiple sequence alignment