Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CDA09_RS20870 Genome accession   NZ_CP021731
Coordinates   4494758..4496200 (+) Length   480 a.a.
NCBI ID   WP_121430402.1    Uniprot ID   -
Organism   Azoarcus sp. DN11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4489758..4501200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDA09_RS20840 (CDA09_20815) thiL 4490502..4491461 (+) 960 WP_121430396.1 thiamine-phosphate kinase -
  CDA09_RS20845 (CDA09_20820) - 4491483..4491962 (+) 480 WP_121430397.1 phosphatidylglycerophosphatase A -
  CDA09_RS20850 (CDA09_20825) - 4491968..4492447 (+) 480 WP_121430398.1 nicotinamide-nucleotide amidohydrolase family protein -
  CDA09_RS20855 (CDA09_20830) recA 4492533..4493567 (+) 1035 WP_121430399.1 recombinase RecA Machinery gene
  CDA09_RS20860 (CDA09_20835) recX 4493582..4494031 (+) 450 WP_121430400.1 recombination regulator RecX -
  CDA09_RS20865 (CDA09_20840) lptB 4494018..4494740 (+) 723 WP_121430401.1 LPS export ABC transporter ATP-binding protein -
  CDA09_RS20870 (CDA09_20845) rpoN/rpoN1 4494758..4496200 (+) 1443 WP_121430402.1 RNA polymerase factor sigma-54 Machinery gene
  CDA09_RS20875 (CDA09_20850) raiA 4496218..4496544 (+) 327 WP_121430403.1 ribosome-associated translation inhibitor RaiA -
  CDA09_RS20880 (CDA09_20855) ptsN 4496681..4497166 (+) 486 WP_121430968.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CDA09_RS20885 (CDA09_20860) hprK 4497135..4498076 (+) 942 WP_121430404.1 HPr(Ser) kinase/phosphatase -
  CDA09_RS20890 (CDA09_20865) ilvA 4498172..4499692 (+) 1521 WP_121430405.1 threonine ammonia-lyase, biosynthetic -
  CDA09_RS20895 (CDA09_20870) - 4499671..4500288 (-) 618 WP_121430406.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 480 a.a.        Molecular weight: 53317.03 Da        Isoelectric Point: 4.7779

>NTDB_id=232933 CDA09_RS20870 WP_121430402.1 4494758..4496200(+) (rpoN/rpoN1) [Azoarcus sp. DN11]
MKPSLQLKISQHLTLTPQLQQSIKLLQLSTLELNQELERFLLENPMLEREDGDGSDFSPGAAQAPSGTTDSGSQSETPAA
SEEPQGESEPANFDEAGEWSSASSGSSNRDDDDTDFQELQAAGTSLRDHLDQQVSLSPLSDRDRALVRFLIEALDEDGYL
NQSLEELLPLLPPELEYDLDDLTIALRHVQHLDPAGIGARNPQECLSLQLRAMLPGATRDLALRIVDEYLDLLAERNFVR
IKRATGCDDDALRAAHALICGLDPHPGSQHSVQETRYILPDVIVRKLRGRWTVSLNPEAMPKLRINSLYASILQQNRGSG
GALTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDYGDVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGV
FELKYFFGSHVATDTGGAASSTAIRALIRQLVEAEDRKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRKTI

Nucleotide


Download         Length: 1443 bp        

>NTDB_id=232933 CDA09_RS20870 WP_121430402.1 4494758..4496200(+) (rpoN/rpoN1) [Azoarcus sp. DN11]
ATGAAACCGTCCCTCCAGCTCAAGATCTCCCAGCACCTCACGCTGACGCCGCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCCACGCTGGAGCTGAACCAGGAGCTGGAACGCTTTCTCCTCGAAAACCCCATGCTCGAACGCGAGGACGGCGACG
GCAGCGACTTTTCGCCCGGCGCAGCGCAGGCCCCGAGCGGCACCACCGATTCCGGCAGCCAGTCAGAAACCCCTGCCGCC
AGCGAGGAGCCCCAGGGCGAGAGCGAACCGGCCAACTTCGACGAGGCCGGCGAGTGGTCGAGCGCGAGCAGCGGTTCGTC
GAACCGCGACGACGACGATACCGACTTCCAGGAGCTGCAGGCCGCCGGCACCAGCCTGCGCGACCACCTCGACCAGCAGG
TGAGCCTGTCGCCGCTCTCCGATCGCGACCGCGCCCTCGTGCGCTTCCTCATCGAGGCGCTCGACGAGGACGGCTACCTC
AACCAGTCGCTCGAGGAACTGCTGCCGCTGCTGCCGCCGGAACTCGAGTACGACCTCGACGACCTCACGATCGCGCTGCG
CCACGTGCAGCACCTCGACCCGGCCGGCATCGGCGCGCGCAATCCGCAGGAATGCCTGAGCCTGCAGCTGCGCGCGATGC
TCCCGGGGGCGACGCGCGACCTCGCGCTCAGGATCGTCGACGAATACCTCGACCTGCTCGCCGAGCGCAATTTCGTGCGC
ATCAAGCGCGCCACCGGCTGCGACGACGACGCGCTGCGCGCCGCGCACGCGCTGATCTGCGGCCTCGACCCGCACCCGGG
CTCGCAGCACTCGGTGCAGGAGACGCGCTACATCCTGCCGGATGTCATCGTGCGCAAGCTGCGCGGCCGCTGGACGGTGT
CGCTCAACCCCGAGGCGATGCCCAAGCTGCGCATCAATTCGCTCTATGCCAGCATCCTGCAGCAGAACCGCGGATCGGGT
GGCGCGCTTACCAGCCAGCTGCAGGAGGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGTTTCGACACGATCCTGCGGGT
TTCGCAGGCCATCGTTGACCAGCAGCGACAGTTCTTCGATTATGGCGATGTGGCAATGCGACCTTTGACGTTGAGGGAGA
TCGCGGACCAGCTCGAGCTGCACGAATCGACGGTCTCGCGAGTCACAACGCAGAAGTTCATGGCCACACCGCGTGGCGTA
TTCGAGTTGAAGTACTTTTTCGGCAGTCACGTTGCCACCGACACCGGTGGGGCGGCTTCCTCCACGGCGATTCGCGCACT
GATTCGCCAGCTGGTGGAGGCCGAAGACAGGAAAAAGCCCCTGTCCGACGCGAAAATTGCCGAATTGCTCGGTCAACAGG
GTATCGTGGTAGCGCGGCGGACGATTGCCAAGTATCGCGAGTCCCTGAATATTCCGCCGGTCAGCCTGCGCAAGACGATC
TGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(323-478)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.489

100

0.579

Multiple sequence alignment