Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   C6N25_RS02085 Genome accession   NZ_CP027340
Coordinates   383485..384294 (-) Length   269 a.a.
NCBI ID   WP_001317326.1    Uniprot ID   -
Organism   Escherichia coli strain 2015C-3121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 378485..389294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6N25_RS02035 - 378672..379238 (-) 567 WP_000287908.1 inovirus-type Gp2 protein -
  C6N25_RS02040 - 379266..379463 (-) 198 WP_001368198.1 inovirus-type Gp2 protein -
  C6N25_RS02045 - 379711..380136 (-) 426 WP_001013303.1 putative zinc ribbon protein -
  C6N25_RS02050 - 380133..380516 (-) 384 WP_000270958.1 putative zinc ribbon protein -
  C6N25_RS02055 - 380775..381344 (-) 570 WP_000221566.1 inovirus Gp2 family protein -
  C6N25_RS30665 - 381544..381744 (+) 201 WP_001297900.1 hypothetical protein -
  C6N25_RS02070 - 382077..382774 (+) 698 WP_094096600.1 IS1-like element IS1A family transposase -
  C6N25_RS02075 - 382791..382991 (-) 201 Protein_387 type II secretion system protein GspC -
  C6N25_RS02080 gspS2 383009..383419 (-) 411 WP_024213407.1 type II secretion system pilot lipoprotein GspS-beta -
  C6N25_RS02085 pilD 383485..384294 (-) 810 WP_001317326.1 prepilin peptidase PppA Machinery gene
  C6N25_RS02090 sslE 384434..389002 (-) 4569 WP_106888736.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29480.21 Da        Isoelectric Point: 8.3821

>NTDB_id=232349 C6N25_RS02085 WP_001317326.1 383485..384294(-) (pilD) [Escherichia coli strain 2015C-3121]
MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
IIKKGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=232349 C6N25_RS02085 WP_001317326.1 383485..384294(-) (pilD) [Escherichia coli strain 2015C-3121]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCATACTGGCTACCGTCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTAATTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTCACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCTCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTGTCACTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTATAAAAAAAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

42.066

100

0.424
  pilD Neisseria gonorrhoeae MS11

39.922

95.911

0.383
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375

Multiple sequence alignment