Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   B4U42_RS09510 Genome accession   NZ_CP021644
Coordinates   1829744..1830325 (+) Length   193 a.a.
NCBI ID   WP_005710402.1    Uniprot ID   A0A143CGR5
Organism   Glaesserella parasuis 29755     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1824744..1835325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4U42_RS09475 (B4U42_09330) gltX 1825488..1826927 (-) 1440 WP_005710409.1 glutamate--tRNA ligase -
  B4U42_RS09495 (B4U42_09350) - 1827633..1827971 (+) 339 WP_005710407.1 hypothetical protein -
  B4U42_RS09500 (B4U42_09355) - 1827975..1828283 (+) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  B4U42_RS09505 (B4U42_09360) - 1828484..1829644 (+) 1161 WP_005710403.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  B4U42_RS09510 (B4U42_09365) pilF 1829744..1830325 (+) 582 WP_005710402.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  B4U42_RS09515 (B4U42_09370) - 1830386..1831321 (+) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  B4U42_RS09520 (B4U42_09375) greB 1831321..1831809 (+) 489 WP_005710400.1 transcription elongation factor GreB -
  B4U42_RS09540 (B4U42_09395) - 1832335..1833633 (+) 1299 WP_005710399.1 FAD-binding oxidoreductase -
  B4U42_RS09545 (B4U42_09400) - 1833718..1834251 (-) 534 WP_005710398.1 TIGR00645 family protein -
  B4U42_RS09550 (B4U42_09405) - 1834340..1834978 (-) 639 WP_005710397.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22324.27 Da        Isoelectric Point: 8.0872

>NTDB_id=232172 B4U42_RS09510 WP_005710402.1 1829744..1830325(+) (pilF) [Glaesserella parasuis 29755]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKLFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=232172 B4U42_RS09510 WP_005710402.1 1829744..1830325(+) (pilF) [Glaesserella parasuis 29755]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTAAATAACTACGGTACCTTTCTGTGCAAACAAAAGCTGTTCGACAAAGCTTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143CGR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

100

100

1
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415

Multiple sequence alignment