Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   C_RS10770 Genome accession   NC_004431
Coordinates   2096023..2097033 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli CFT073     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2091023..2102033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C_RS10750 (c2270) mepM 2091990..2093312 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  C_RS10755 (c2271) znuA 2093328..2094260 (-) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  C_RS10760 (c2272) znuC 2094339..2095094 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  C_RS10765 (c2273) znuB 2095091..2095876 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  C_RS10770 (c2274) ruvB 2096023..2097033 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  C_RS10775 (c2275) ruvA 2097042..2097653 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  C_RS28620 yobI 2097792..2097857 (-) 66 WP_072123646.1 stress response small protein YobI -
  C_RS10780 (c2276) yebB 2097929..2098531 (+) 603 WP_001024947.1 YebB family permuted papain-like enzyme -
  C_RS10785 (c2277) ruvC 2098533..2099054 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  C_RS10790 (c2278) yebC 2099089..2099829 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  C_RS10795 (c2279) nudB 2099858..2100310 (-) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=23139 C_RS10770 WP_000568522.1 2096023..2097033(-) (ruvB) [Escherichia coli CFT073]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=23139 C_RS10770 WP_000568522.1 2096023..2097033(-) (ruvB) [Escherichia coli CFT073]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment