Detailed information NTG0229618

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: CA924_RS03560
• Genome accession: NZ_CP021281
• Coordinates: 733530..733979 (-)
• Length: 149 a.a.
• NCBI ID: WP_010946364.1
• Uniprot ID: Q5ZXV4
• Organism: Legionella pneumophila subsp. pneumophila strain Flint
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 728530..738979

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CA924_RS03540	-	728805..729197 (-)	393	WP_010946360.1	hypothetical protein	-
CA924_RS03545	-	729334..729711 (+)	378	WP_010946361.1	hypothetical protein	-
CA924_RS03550	-	729912..731219 (+)	1308	WP_011213222.1	tetratricopeptide repeat protein	-
CA924_RS03555	comEC	731245..733452 (-)	2208	WP_010946363.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CA924_RS03560	pilE	733530..733979 (-)	450	WP_010946364.1	type IV pilin protein	Machinery gene
CA924_RS03565	-	733991..737500 (-)	3510	WP_011213223.1	PilC/PilY family type IV pilus protein	-
CA924_RS03570	-	737513..738025 (-)	513	WP_010946366.1	pilus assembly protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16296.57 Da
• Isoelectric Point: 7.6922

>NTDB_id=229618 CA924_RS03560 WP_010946364.1 733530..733979(-) (pilE) [Legionella pneumophila subsp. pneumophila strain Flint]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=229618 CA924_RS03560 WP_010946364.1 733530..733979(-) (pilE) [Legionella pneumophila subsp. pneumophila strain Flint]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGCATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAATCCCGTCGTGCCGATGCTC
ACGCCACATTGACACAAGATCAAATTATTTTAGAACGCTGTTATTCACAGAATTTTTCTTATGCTGCGGCGTGTGGCGCC
TTACCAGCATTTCCTCAAACAACGCCGAACGGGTACTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACTGCAACCCCTGTTGGAACTCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATTAACCAGGCCAATGTAAAAACAG
CAGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: Q5ZXV4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	100	100	1
pilE	Legionella pneumophila strain ERS1305867	100	100	1