Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B5M25_RS08170 Genome accession   NZ_CP021171
Coordinates   1621856..1622542 (-) Length   228 a.a.
NCBI ID   WP_110228646.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CHU15-056     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1616856..1627542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5M25_RS08150 (B5M25_08045) rpsT 1617770..1618021 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  B5M25_RS08155 (B5M25_08050) holA 1618066..1619040 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  B5M25_RS08160 (B5M25_08055) comEC 1619097..1621298 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B5M25_RS08165 (B5M25_08060) - 1621303..1621764 (-) 462 WP_000439693.1 ComE operon protein 2 -
  B5M25_RS08170 (B5M25_08065) comEA 1621856..1622542 (-) 687 WP_110228646.1 ComEA family DNA-binding protein Machinery gene
  B5M25_RS08175 (B5M25_08070) - 1622582..1623298 (-) 717 WP_000084830.1 class I SAM-dependent methyltransferase -
  B5M25_RS08180 (B5M25_08075) rsfS 1623301..1623654 (-) 354 WP_001088020.1 ribosome silencing factor -
  B5M25_RS08185 (B5M25_08080) yqeK 1623655..1624239 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B5M25_RS08190 (B5M25_08085) nadD 1624229..1624798 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  B5M25_RS08195 (B5M25_08090) yhbY 1624801..1625091 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  B5M25_RS08200 (B5M25_08095) aroE 1625095..1625901 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  B5M25_RS08205 (B5M25_08100) yqeH 1625915..1627015 (-) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25927.67 Da        Isoelectric Point: 9.6751

>NTDB_id=228840 B5M25_RS08170 WP_110228646.1 1621856..1622542(-) (comEA) [Staphylococcus aureus strain CHU15-056]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQNTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=228840 B5M25_RS08170 WP_110228646.1 1621856..1622542(-) (comEA) [Staphylococcus aureus strain CHU15-056]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAACACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

99.561

100

0.996
  comEA Staphylococcus aureus MW2

96.93

100

0.969

Multiple sequence alignment