Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   B9N48_RS12415 Genome accession   NZ_CP021169
Coordinates   2384301..2385338 (-) Length   345 a.a.
NCBI ID   WP_086344083.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2379301..2390338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9N48_RS12365 tasA 2379398..2380183 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  B9N48_RS12370 sipW 2380247..2380819 (-) 573 WP_086344613.1 signal peptidase I SipW -
  B9N48_RS12375 tapA 2380803..2381564 (-) 762 WP_086344079.1 amyloid fiber anchoring/assembly protein TapA -
  B9N48_RS12380 yqzG 2381837..2382163 (+) 327 WP_086344614.1 YqzG/YhdC family protein -
  B9N48_RS12385 spoIITA 2382205..2382384 (-) 180 WP_003230176.1 YqzE family protein -
  B9N48_RS12390 comGG 2382455..2382829 (-) 375 WP_086344080.1 ComG operon protein ComGG Machinery gene
  B9N48_RS12395 comGF 2382830..2383213 (-) 384 WP_024573389.1 ComG operon protein ComGF Machinery gene
  B9N48_RS12400 comGE 2383239..2383586 (-) 348 WP_086344081.1 ComG operon protein 5 Machinery gene
  B9N48_RS12405 comGD 2383570..2384001 (-) 432 WP_086344082.1 comG operon protein ComGD Machinery gene
  B9N48_RS12410 comGC 2383991..2384287 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  B9N48_RS12415 comGB 2384301..2385338 (-) 1038 WP_086344083.1 comG operon protein ComGB Machinery gene
  B9N48_RS12420 comGA 2385325..2386395 (-) 1071 WP_070547533.1 competence protein ComGA Machinery gene
  B9N48_RS12425 - 2386606..2386803 (-) 198 WP_032726162.1 hypothetical protein -
  B9N48_RS12430 corA 2386805..2387758 (-) 954 WP_072173930.1 magnesium transporter CorA -
  B9N48_RS12435 yqhB 2387901..2389229 (+) 1329 WP_014480261.1 hemolysin family protein -
  B9N48_RS12440 rsbRD 2389282..2390118 (-) 837 WP_021480027.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39676.12 Da        Isoelectric Point: 9.7153

>NTDB_id=228777 B9N48_RS12415 WP_086344083.1 2384301..2385338(-) (comGB) [Bacillus subtilis strain TLO3]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTGTVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=228777 B9N48_RS12415 WP_086344083.1 2384301..2385338(-) (comGB) [Bacillus subtilis strain TLO3]
ATGAAAAAGATTAGAAAAGTCTGGTCATTAAAGGATCAAGCCAGGTTATTAAAGCGGCTCGGTGAAATGACTGCGGGGGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGGTACGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.071

93.623

0.928

Multiple sequence alignment