Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   B9N48_RS12420 Genome accession   NZ_CP021169
Coordinates   2385325..2386395 (-) Length   356 a.a.
NCBI ID   WP_070547533.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2380325..2391395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9N48_RS12375 tapA 2380803..2381564 (-) 762 WP_086344079.1 amyloid fiber anchoring/assembly protein TapA -
  B9N48_RS12380 yqzG 2381837..2382163 (+) 327 WP_086344614.1 YqzG/YhdC family protein -
  B9N48_RS12385 spoIITA 2382205..2382384 (-) 180 WP_003230176.1 YqzE family protein -
  B9N48_RS12390 comGG 2382455..2382829 (-) 375 WP_086344080.1 ComG operon protein ComGG Machinery gene
  B9N48_RS12395 comGF 2382830..2383213 (-) 384 WP_024573389.1 ComG operon protein ComGF Machinery gene
  B9N48_RS12400 comGE 2383239..2383586 (-) 348 WP_086344081.1 ComG operon protein 5 Machinery gene
  B9N48_RS12405 comGD 2383570..2384001 (-) 432 WP_086344082.1 comG operon protein ComGD Machinery gene
  B9N48_RS12410 comGC 2383991..2384287 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  B9N48_RS12415 comGB 2384301..2385338 (-) 1038 WP_086344083.1 comG operon protein ComGB Machinery gene
  B9N48_RS12420 comGA 2385325..2386395 (-) 1071 WP_070547533.1 competence protein ComGA Machinery gene
  B9N48_RS12425 - 2386606..2386803 (-) 198 WP_032726162.1 hypothetical protein -
  B9N48_RS12430 corA 2386805..2387758 (-) 954 WP_072173930.1 magnesium transporter CorA -
  B9N48_RS12435 yqhB 2387901..2389229 (+) 1329 WP_014480261.1 hemolysin family protein -
  B9N48_RS12440 rsbRD 2389282..2390118 (-) 837 WP_021480027.1 RsbT co-antagonist protein RsbRD -
  B9N48_RS12450 mgsR 2390342..2390722 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  B9N48_RS12455 yqgY 2390954..2391199 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40444.57 Da        Isoelectric Point: 8.8402

>NTDB_id=228778 B9N48_RS12420 WP_070547533.1 2385325..2386395(-) (comGA) [Bacillus subtilis strain TLO3]
MDSIENVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=228778 B9N48_RS12420 WP_070547533.1 2385325..2386395(-) (comGA) [Bacillus subtilis strain TLO3]
TTGGATTCAATAGAAAATGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCTGCAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCCATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997

Multiple sequence alignment