Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B6257_RS11740 Genome accession   NZ_CP021011
Coordinates   2257778..2258215 (-) Length   145 a.a.
NCBI ID   WP_071392144.1    Uniprot ID   -
Organism   Bacillus velezensis strain GFP-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2252778..2263215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6257_RS20145 - 2253372..2253500 (-) 129 Protein_2251 hypothetical protein -
  B6257_RS11705 (B6257_11640) - 2253645..2254847 (+) 1203 WP_071392147.1 GTP-binding protein -
  B6257_RS11710 (B6257_11645) - 2254912..2256048 (+) 1137 WP_071392146.1 zinc-dependent alcohol dehydrogenase -
  B6257_RS11715 (B6257_11650) - 2256063..2256497 (+) 435 WP_021494203.1 RDD family protein -
  B6257_RS11720 (B6257_11655) - 2256614..2256889 (+) 276 WP_373369498.1 YckD family protein -
  B6257_RS19870 - 2256993..2257184 (+) 192 WP_071392145.1 family 1 glycosylhydrolase -
  B6257_RS11730 (B6257_11665) - 2257109..2257338 (+) 230 Protein_2257 family 1 glycosylhydrolase -
  B6257_RS11735 (B6257_11670) nin/comJ 2257357..2257755 (-) 399 WP_101562687.1 competence protein ComJ Regulator
  B6257_RS11740 (B6257_11675) nucA/comI 2257778..2258215 (-) 438 WP_071392144.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  B6257_RS11745 (B6257_11680) hxlB 2258576..2259133 (-) 558 WP_071392143.1 6-phospho-3-hexuloisomerase -
  B6257_RS11750 (B6257_11685) hxlA 2259130..2259765 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  B6257_RS11755 (B6257_11690) - 2259997..2260359 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16099.43 Da        Isoelectric Point: 9.4115

>NTDB_id=227821 B6257_RS11740 WP_071392144.1 2257778..2258215(-) (nucA/comI) [Bacillus velezensis strain GFP-2]
MKTLKALLLVLIIIAGAVAALIKGDLFSNNEQTVRKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=227821 B6257_RS11740 WP_071392144.1 2257778..2258215(-) (nucA/comI) [Bacillus velezensis strain GFP-2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAAATAACGAGCAGACCGTTCGGAAGAGTGATTACGATAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGG
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634

Multiple sequence alignment