Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   B9L64_RS10240 Genome accession   NZ_CP020915
Coordinates   2005058..2006449 (-) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis strain 50-1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2000058..2011449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9L64_RS10205 flgL 2000229..2001125 (-) 897 WP_003228004.1 flagellar hook-associated protein FlgL -
  B9L64_RS10210 flgK 2001136..2002659 (-) 1524 WP_110109495.1 flagellar hook-associated protein FlgK -
  B9L64_RS10215 flgN 2002678..2003160 (-) 483 WP_003227999.1 flagellar protein FlgN -
  B9L64_RS10220 flgM 2003176..2003442 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  B9L64_RS10225 yvyF 2003523..2003942 (-) 420 WP_110109496.1 TIGR03826 family flagellar region protein -
  B9L64_RS10230 comFC 2004016..2004738 (-) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  B9L64_RS10235 comFB 2004702..2004998 (-) 297 WP_003227989.1 late competence protein ComFB -
  B9L64_RS10240 comFA 2005058..2006449 (-) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  B9L64_RS10245 fakBA 2006555..2007400 (-) 846 WP_003244125.1 DegV family protein -
  B9L64_RS10250 degU 2007498..2008187 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  B9L64_RS10255 degS 2008270..2009427 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  B9L64_RS10260 yvyE 2009644..2010297 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=227085 B9L64_RS10240 WP_003243962.1 2005058..2006449(-) (comFA) [Bacillus subtilis strain 50-1]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=227085 B9L64_RS10240 WP_003243962.1 2005058..2006449(-) (comFA) [Bacillus subtilis strain 50-1]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment