Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   B9K09_RS18310 Genome accession   NZ_CP020892
Coordinates   3959858..3961456 (+) Length   532 a.a.
NCBI ID   WP_087518167.1    Uniprot ID   A0A1Y0KVL0
Organism   Pseudomonas sp. M30-35     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3954858..3966456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9K09_RS18290 (B9K09_18280) pgeF 3956556..3957293 (-) 738 WP_087518163.1 peptidoglycan editing factor PgeF -
  B9K09_RS18295 (B9K09_18285) rluD 3957290..3958264 (-) 975 WP_087518164.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  B9K09_RS18300 (B9K09_18290) - 3958416..3959411 (+) 996 WP_087518165.1 outer membrane protein assembly factor BamD -
  B9K09_RS18305 (B9K09_18295) - 3959629..3959868 (+) 240 WP_087518166.1 PP0621 family protein -
  B9K09_RS18310 (B9K09_18300) pilS 3959858..3961456 (+) 1599 WP_087518167.1 nitrogen regulation protein NR(II) Regulator
  B9K09_RS18315 (B9K09_18305) pilR 3961453..3962790 (+) 1338 WP_087518168.1 sigma-54 dependent transcriptional regulator Regulator
  B9K09_RS18320 (B9K09_18310) - 3962847..3963821 (-) 975 WP_087516234.1 IS110 family transposase -
  B9K09_RS18325 (B9K09_18315) thiO 3964176..3965261 (-) 1086 WP_087518169.1 glycine oxidase ThiO -
  B9K09_RS18330 (B9K09_18320) - 3965315..3965833 (+) 519 WP_087518170.1 GspH/FimT family pseudopilin -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58998.41 Da        Isoelectric Point: 8.1951

>NTDB_id=226941 B9K09_RS18310 WP_087518167.1 3959858..3961456(+) (pilS) [Pseudomonas sp. M30-35]
MVNDALALGGTQGIRILKLYHLYRLIIGLLLVLLISSEMDVELLEMTHATLFRNISWFYLIFNVLVTLAVQQPKRSMQVV
SLAMVDVILLSCLFYAAGGTPSGIGNLLIVAVAISNILLRGRIGIFIAATAAVGLIYLTFYLSLSRPAAVSQYIQAGALG
ALCFAAALFVQGITRRLHNSEQLASARAADVASLEILNALIIERMRTGILVLDNMHRVLLANHGALTLLGSDGLAGKILD
PHTPELVKRLQQWRQNPAMRPASLQAFADGPIVQPSFVALQRGQQRHILVFLEDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELTQPDQRLAQIIQDHSHRMNLVIENVLQLSRRRQSEPQLLDLKYWLHRFASEFRSTMPAN
QTLHLDTQVGTIHTRMDPNQLTQVLSNLVQNGLRYSAQKNPRGQVWLQLFREPDRDLPVLEILDDGPGVSPEQNQNIFEP
FFTTEAKGTGLGLYISRELCESNQAHIDYKPREEGGSCFRITFAHASKLSLQ

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=226941 B9K09_RS18310 WP_087518167.1 3959858..3961456(+) (pilS) [Pseudomonas sp. M30-35]
GTGGTCAATGACGCTCTGGCTCTGGGCGGTACCCAGGGCATACGAATTCTCAAGCTGTATCACCTATACCGGCTGATTAT
CGGCCTGCTGTTGGTACTGCTGATTTCAAGCGAAATGGACGTAGAGTTGCTGGAGATGACCCATGCCACTCTGTTTCGCA
ATATCAGTTGGTTTTATCTGATATTCAATGTGCTGGTTACCCTTGCAGTCCAGCAACCCAAGCGTTCCATGCAGGTTGTC
AGCCTGGCGATGGTCGACGTTATTCTGTTGTCGTGCCTGTTTTATGCGGCTGGCGGTACGCCCAGCGGTATCGGCAACCT
ACTGATCGTCGCAGTCGCCATCTCTAATATCCTGCTACGCGGGCGCATCGGTATTTTTATTGCCGCCACCGCCGCCGTCG
GCTTGATCTACCTGACTTTCTACCTGAGCCTCAGCCGCCCCGCCGCTGTCAGCCAATATATCCAAGCTGGCGCCCTTGGA
GCACTTTGCTTCGCGGCAGCCCTGTTTGTTCAAGGAATTACCCGACGCCTGCATAACAGTGAACAGTTAGCCAGCGCGCG
CGCAGCAGACGTTGCCAGCCTGGAAATCCTGAACGCGCTGATCATTGAGAGAATGCGCACAGGCATTCTGGTATTGGACA
ACATGCACCGTGTGTTACTGGCCAACCACGGGGCGCTGACACTGCTCGGTAGTGACGGCCTAGCCGGTAAGATTCTTGAT
CCGCACACCCCGGAACTGGTTAAACGCTTGCAGCAGTGGCGTCAAAACCCGGCGATGCGCCCTGCCAGTCTGCAAGCGTT
TGCAGACGGCCCCATCGTGCAGCCAAGCTTTGTCGCTCTGCAACGCGGCCAACAACGCCACATATTAGTGTTTCTGGAAG
ATATTTCGCAGATAGCCCAACAAGCCCAGCAGCTCAAACTCGCTTCTCTCGGTCGCCTAACAGCCGGTATTGCTCACGAA
ATCCGCAACCCACTCGGCGCGATCAGCCATGCCGCGCAGTTGCTCCAGGAGTCTGAAGAGCTCACCCAGCCAGACCAGCG
ACTGGCACAAATCATTCAAGATCACTCGCACCGTATGAACCTGGTGATTGAAAACGTTCTACAACTTTCACGCAGGCGCC
AGTCGGAACCACAACTTCTCGACCTCAAGTACTGGCTGCACCGCTTTGCCAGCGAGTTCCGCAGCACCATGCCCGCCAAC
CAAACGCTGCACCTGGACACCCAGGTTGGCACCATCCATACACGTATGGACCCCAATCAGCTGACCCAGGTACTGAGTAA
CCTGGTCCAGAACGGCTTGCGCTACAGCGCACAAAAGAACCCACGGGGTCAGGTTTGGTTACAACTGTTCCGCGAGCCCG
ATCGCGACCTTCCAGTGCTGGAAATCCTTGATGACGGGCCAGGTGTTTCGCCGGAGCAGAATCAGAATATCTTCGAGCCC
TTCTTCACCACCGAAGCCAAGGGCACCGGCCTGGGCCTGTATATTTCTCGTGAACTCTGCGAGAGCAACCAAGCCCACAT
TGACTATAAACCCCGCGAAGAGGGTGGCAGCTGCTTTCGCATCACCTTCGCCCACGCCAGCAAACTGAGCTTACAATGA

Domains

Predicted by InterproScan.

PAS domain (PAS_8)

(201-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0KVL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

64.906

99.624

0.647

Multiple sequence alignment