Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B9C53_RS08340 Genome accession   NZ_CP020874
Coordinates   1829387..1829824 (+) Length   145 a.a.
NCBI ID   WP_038456921.1    Uniprot ID   -
Organism   Bacillus velezensis strain GYL4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1824387..1834824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9C53_RS08325 (B9C53_08315) - 1827247..1827609 (-) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -
  B9C53_RS08330 (B9C53_08320) hxlA 1827841..1828476 (+) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  B9C53_RS08335 (B9C53_08325) hxlB 1828473..1829030 (+) 558 WP_038456922.1 6-phospho-3-hexuloisomerase -
  B9C53_RS08340 (B9C53_08330) nucA/comI 1829387..1829824 (+) 438 WP_038456921.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  B9C53_RS08345 (B9C53_08335) nin/comJ 1829845..1830243 (+) 399 WP_038456919.1 competence protein ComJ Regulator
  B9C53_RS08350 (B9C53_08340) - 1830398..1830676 (-) 279 WP_263612330.1 YckD family protein -
  B9C53_RS08355 (B9C53_08345) - 1830793..1831227 (-) 435 WP_094031491.1 RDD family protein -
  B9C53_RS08360 (B9C53_08350) - 1831242..1832378 (-) 1137 WP_094031490.1 zinc-dependent alcohol dehydrogenase -
  B9C53_RS08365 (B9C53_08355) - 1832443..1833645 (-) 1203 WP_069007411.1 GTP-binding protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16099.38 Da        Isoelectric Point: 8.4850

>NTDB_id=226766 B9C53_RS08340 WP_038456921.1 1829387..1829824(+) (nucA/comI) [Bacillus velezensis strain GYL4]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKNDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=226766 B9C53_RS08340 WP_038456921.1 1829387..1829824(+) (nucA/comI) [Bacillus velezensis strain GYL4]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAATGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment