Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   B7437_RS08260 Genome accession   NZ_CP020741
Coordinates   1649190..1649687 (-) Length   165 a.a.
NCBI ID   WP_001838632.1    Uniprot ID   A0A6A9GZK7
Organism   Staphylococcus aureus strain HZW450     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644190..1654687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7437_RS08235 (B7437_08305) gcvPB 1644352..1645824 (-) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  B7437_RS08240 (B7437_08310) gcvPA 1645817..1647163 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  B7437_RS08245 (B7437_08315) gcvT 1647183..1648274 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  B7437_RS08250 (B7437_08320) - 1648433..1648957 (-) 525 WP_001015116.1 shikimate kinase -
  B7437_RS08255 (B7437_08325) - 1648947..1649093 (-) 147 WP_001789879.1 hypothetical protein -
  B7437_RS08260 (B7437_08330) comGF 1649190..1649687 (-) 498 WP_001838632.1 competence type IV pilus minor pilin ComGF Machinery gene
  B7437_RS08265 (B7437_08335) comGE 1649605..1649904 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  B7437_RS08270 (B7437_08340) comGD 1649891..1650337 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  B7437_RS08275 (B7437_08345) comGC 1650315..1650626 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  B7437_RS08280 (B7437_08350) comGB 1650640..1651710 (-) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  B7437_RS08285 (B7437_08355) comGA 1651682..1652656 (-) 975 WP_000697218.1 competence type IV pilus ATPase ComGA Machinery gene
  B7437_RS08290 (B7437_08360) - 1652708..1653331 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  B7437_RS08295 (B7437_08365) - 1653328..1653657 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  B7437_RS08300 (B7437_08370) - 1653657..1654643 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19184.87 Da        Isoelectric Point: 10.2192

>NTDB_id=225628 B7437_RS08260 WP_001838632.1 1649190..1649687(-) (comGF) [Staphylococcus aureus strain HZW450]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIENREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=225628 B7437_RS08260 WP_001838632.1 1649190..1649687(-) (comGF) [Staphylococcus aureus strain HZW450]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCTCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAAATAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A9GZK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

96.364

100

0.964
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment