Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B7474_RS02460 Genome accession   NZ_CP020714
Coordinates   506369..507055 (-) Length   228 a.a.
NCBI ID   WP_072361794.1    Uniprot ID   -
Organism   Staphylococcus aureus strain K18     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501369..512055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7474_RS02440 (B7474_02430) rpsT 502283..502534 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  B7474_RS02445 (B7474_02435) holA 502579..503553 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  B7474_RS02450 (B7474_02440) comEC 503610..505811 (-) 2202 WP_025174327.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B7474_RS02455 (B7474_02445) - 505816..506277 (-) 462 WP_000439693.1 ComE operon protein 2 -
  B7474_RS02460 (B7474_02450) comEA 506369..507055 (-) 687 WP_072361794.1 ComEA family DNA-binding protein Machinery gene
  B7474_RS02465 (B7474_02455) - 507095..507811 (-) 717 WP_000084822.1 class I SAM-dependent DNA methyltransferase -
  B7474_RS02470 (B7474_02460) rsfS 507814..508167 (-) 354 WP_001088020.1 ribosome silencing factor -
  B7474_RS02475 (B7474_02465) yqeK 508168..508752 (-) 585 WP_001019326.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B7474_RS02480 (B7474_02470) nadD 508742..509311 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  B7474_RS02485 (B7474_02475) yhbY 509314..509604 (-) 291 WP_000627815.1 ribosome assembly RNA-binding protein YhbY -
  B7474_RS02490 (B7474_02480) aroE 509608..510414 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  B7474_RS02495 (B7474_02485) yqeH 510428..511528 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=225492 B7474_RS02460 WP_072361794.1 506369..507055(-) (comEA) [Staphylococcus aureus strain K18]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=225492 B7474_RS02460 WP_072361794.1 506369..507055(-) (comEA) [Staphylococcus aureus strain K18]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.678

99.561

0.982
  comEA Staphylococcus aureus N315

97.797

99.561

0.974

Multiple sequence alignment