Detailed information NTG0224817

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B7L46_RS21190
• Genome accession: NZ_CP020597
• Coordinates: 2016609..2017469 (-)
• Length: 286 a.a.
• NCBI ID: WP_001152283.1
• Uniprot ID: A0A009QK25
• Organism: Acinetobacter baumannii strain HWBA8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2011609..2022469

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L46_RS10620 (B7L46_10620)	-	2012500..2013810 (+)	1311	WP_000753283.1	tetratricopeptide repeat protein	-
B7L46_RS10625 (B7L46_10625)	-	2013887..2014213 (+)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
B7L46_RS10630 (B7L46_10630)	rlmB	2014315..2015064 (+)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
B7L46_RS10635 (B7L46_10635)	-	2015100..2016014 (+)	915	WP_000075020.1	DMT family transporter	-
B7L46_RS10640 (B7L46_10640)	coaE	2016011..2016607 (-)	597	WP_000874125.1	dephospho-CoA kinase	-
B7L46_RS21190 (B7L46_10645)	pilD	2016609..2017469 (-)	861	WP_001152283.1	prepilin peptidase	Machinery gene
B7L46_RS10650 (B7L46_10650)	pilC	2017469..2018695 (-)	1227	WP_000279216.1	type II secretion system F family protein	Machinery gene
B7L46_RS10655 (B7L46_10655)	pilB	2018724..2020436 (-)	1713	WP_001274992.1	type IV-A pilus assembly ATPase PilB	Machinery gene
B7L46_RS10660 (B7L46_10660)	tpiA	2020727..2021521 (+)	795	WP_000016932.1	triose-phosphate isomerase	-
B7L46_RS10665 (B7L46_10665)	secG	2021534..2021863 (+)	330	WP_000555570.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32144.59 Da
• Isoelectric Point: 7.1041

>NTDB_id=224817 B7L46_RS21190 WP_001152283.1 2016609..2017469(-) (pilD) [Acinetobacter baumannii strain HWBA8]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPRMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPVIELLTMLCSLVVVMMFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=224817 B7L46_RS21190 WP_001152283.1 2016609..2017469(-) (pilD) [Acinetobacter baumannii strain HWBA8]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTAATTTACCGCACGCCAAGAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGTTACTCA
ATCCTGAGCAACCGATTATTGATCATGAGAAGTTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCAATCAG
TATTCGCTATCCAGTCATCGAACTACTCACCATGCTATGTTCATTAGTAGTCGTCATGATGTTTGGCCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACTTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCTTACCTTTAGCTGCGCTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGTGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

• Type IV leader peptidase family (Peptidase_A24) (138-247)

3D structure

• AlphaFold DB: A0A009QK25

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	98.601	100	0.986
pilD	Acinetobacter baumannii D1279779	98.252	100	0.983
pilD	Vibrio cholerae strain A1552	53.676	95.105	0.51
pilD	Vibrio campbellii strain DS40M4	52.399	94.755	0.497
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441