Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   B7L44_RS02265 Genome accession   NZ_CP020588
Coordinates   462491..463231 (+) Length   246 a.a.
NCBI ID   WP_016803752.1    Uniprot ID   A0A2L1VMD3
Organism   Acinetobacter nosocomialis strain SSA3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457491..468231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7L44_RS02250 (B7L44_02250) ponA 458072..460630 (-) 2559 WP_031982123.1 penicillin-binding protein PBP1a -
  B7L44_RS02255 (B7L44_02255) comM 460792..461850 (+) 1059 WP_004711654.1 pilus assembly protein PilM Machinery gene
  B7L44_RS02260 (B7L44_02260) pilN 461850..462494 (+) 645 WP_006581060.1 PilN domain-containing protein Machinery gene
  B7L44_RS02265 (B7L44_02265) pilO 462491..463231 (+) 741 WP_016803752.1 type 4a pilus biogenesis protein PilO Machinery gene
  B7L44_RS02270 (B7L44_02270) pilP 463242..463769 (+) 528 WP_006582542.1 pilus assembly protein PilP Machinery gene
  B7L44_RS02275 (B7L44_02275) pilQ 463832..465997 (+) 2166 WP_004884727.1 type IV pilus secretin PilQ family protein Machinery gene
  B7L44_RS02280 (B7L44_02280) aroK 466009..466578 (+) 570 WP_002051689.1 shikimate kinase AroK -
  B7L44_RS02285 (B7L44_02285) aroB 466607..467701 (+) 1095 WP_083008232.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27639.43 Da        Isoelectric Point: 4.9673

>NTDB_id=224538 B7L44_RS02265 WP_016803752.1 462491..463231(+) (pilO) [Acinetobacter nosocomialis strain SSA3]
MSQDELQELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
GNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSISALPRIVTMHDFVVEAAQAKDSKSDIPVLNYSIKAKTYRYIGVTEASAQPAKAAAATAPTS
PNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=224538 B7L44_RS02265 WP_016803752.1 462491..463231(+) (pilO) [Acinetobacter nosocomialis strain SSA3]
ATGAGTCAAGATGAACTGCAAGAATTATCATTAGAGCAATCAGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTTGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCCCAAGCTCAGGAA
GGTAATTTATTAAATGAATTTCGAGAAAAAGACTCGAAATTACGTAATCTTCAGCAGTATCAAGTGCAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAATTAGAACAATTGCCGAAAGAAACAGAGATTCCAAGTTTAGTTGAGGATATTAACTTAA
CTGGTGTGAACTCTGGATTGAAATTTAAAAATATTCGTTTAGAGGATGAAGTAAAGCAGGAAATTTTTATTGAACAACCA
ATTGCCATCGAAGCAACAGGCGACTATCACGCTTTCGGTGCTTTCGTAAGTAGTATCTCTGCTTTGCCACGTATTGTGAC
GATGCATGACTTTGTTGTAGAGGCTGCTCAAGCTAAAGATAGTAAGTCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CCAAAACCTATCGATATATAGGAGTAACAGAGGCATCGGCACAACCAGCTAAGGCTGCAGCGGCGACTGCACCAACTAGC
CCTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1VMD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.024

100

0.89
  comO Acinetobacter baylyi ADP1

71.369

97.967

0.699

Multiple sequence alignment