Detailed information NTG0224502

Bioinformatically predicted

Overview

• Name: pilO
• Type: Machinery gene
• Locus tag: B7L36_RS04410
• Genome accession: NZ_CP020586
• Coordinates: 798249..798989 (+)
• Length: 246 a.a.
• NCBI ID: WP_000076101.1
• Uniprot ID: N9LH62
• Organism: Acinetobacter baumannii strain CBA7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	799649..800857	798249..798989	flank	660

Gene organization within MGE regions

Location: 798249..800857

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L36_RS04410 (B7L36_04410)	pilO	798249..798989 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
B7L36_RS04415 (B7L36_04415)	pilP	799000..799527 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
B7L36_RS04420 (B7L36_04420)	-	799649..800857 (+)	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-

Sequence

Protein

• Length: 246 a.a.
• Molecular weight: 27819.59 Da
• Isoelectric Point: 4.8357

>NTDB_id=224502 B7L36_RS04410 WP_000076101.1 798249..798989(+) (pilO) [Acinetobacter baumannii strain CBA7]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide

• Length: 741 bp

>NTDB_id=224502 B7L36_RS04410 WP_000076101.1 798249..798989(+) (pilO) [Acinetobacter baumannii strain CBA7]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

• Pilus assembly protein, PilO (PilO) (73-220)

3D structure

• AlphaFold DB: N9LH62

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilO	Acinetobacter baumannii D1279779	100	100	1
comO	Acinetobacter baylyi ADP1	69.421	98.374	0.683