Detailed information NTG0223046

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: A6J74_RS11200
• Genome accession: NZ_CP020437
• Coordinates: 1830878..1834603 (-)
• Length: 1241 a.a.
• NCBI ID: WP_000572319.1
• Uniprot ID: A0AAP8JW19
• Organism: Bacillus sp. FDAARGOS_235
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1825878..1839603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A6J74_RS11150 (A6J74_11160)	-	1826762..1827043 (+)	282	WP_080609500.1	hypothetical protein	-
A6J74_RS11155 (A6J74_11165)	-	1827079..1827978 (-)	900	WP_001213067.1	fumarylacetoacetate hydrolase family protein	-
A6J74_RS11160 (A6J74_11170)	-	1828225..1828401 (+)	177	WP_000462849.1	aspartyl-phosphate phosphatase Spo0E family protein	-
A6J74_RS11165 (A6J74_11175)	gerPA	1828501..1828722 (+)	222	WP_001111187.1	spore germination protein GerPA	-
A6J74_RS11170 (A6J74_11180)	gerPB	1828737..1828943 (+)	207	WP_001012514.1	spore germination protein GerPB	-
A6J74_RS11175 (A6J74_11185)	gerPC	1829012..1829626 (+)	615	WP_001070753.1	spore germination protein GerPC	-
A6J74_RS11180 (A6J74_11190)	gerPD	1829633..1829827 (+)	195	WP_001052804.1	spore germination protein GerPD	-
A6J74_RS11185 (A6J74_11195)	-	1829843..1830229 (+)	387	WP_000902321.1	spore germination protein GerPE	-
A6J74_RS11190 (A6J74_11200)	gerPF	1830272..1830487 (+)	216	WP_001141566.1	spore germination protein GerPF	-
A6J74_RS11195 (A6J74_11205)	-	1830576..1830854 (-)	279	WP_000224035.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
A6J74_RS11200 (A6J74_11210)	addA	1830878..1834603 (-)	3726	WP_000572319.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
A6J74_RS11205 (A6J74_11215)	addB	1834600..1838115 (-)	3516	WP_000058641.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
A6J74_RS11210 (A6J74_11220)	lepB	1838232..1838795 (-)	564	WP_000751915.1	signal peptidase I	-
A6J74_RS11215 (A6J74_11225)	-	1838978..1839571 (-)	594	WP_000347508.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142735.89 Da
• Isoelectric Point: 4.8330

>NTDB_id=223046 A6J74_RS11200 WP_000572319.1 1830878..1834603(-) (addA) [Bacillus sp. FDAARGOS_235]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGMLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVKLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENDEVKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPIGEDVAAELLCIQQTEEEVLEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHTVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=223046 A6J74_RS11200 WP_000572319.1 1830878..1834603(-) (addA) [Bacillus sp. FDAARGOS_235]
ATGATAGAGAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGACG
TGACATTTTAGTCGCAGCAGCAGCTGGATCGGGAAAAACAGCAGTATTAGTTGAACGTATGATTAAAAAGATTATTAATG
AGGAAAATCCAGTCGATGTCGATCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTACTAATTGATGAGCCAGGATCTCAACATGTAAGAAAACAGCTGAGCCTATTAAATAAAGC
ATCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGGTATTACTACATGCTGGATGTTGATCCTCGTTTTC
GTATTGCAAATCAAACAGAAAATGAGTTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGCTATACGAGTGACCGTAGTGATGATGACTTACAAAGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACCTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCGTTGAAACCCTGCAAGCGGATGCAGCTTTACT
TGGAATGTTATCATCAGCAGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTCGTAAAAGTCTTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAACAAAGTGAAAATGATGAAGTGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCAGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTACCGTTTCCGACTAGCAG
AACCAGGCTTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATCGGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAATAGGTGAAGATGTAGCAGCTGAATTATTATGCA
TTCAGCAAACGGAAGAAGAAGTACTAGAGGGTGAAGAAGGTACGGAAGTCGAAAAGGCGCAACTGGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGGTCATTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAAGAACAAGAAC
TTCATGAAAAACTAGAATGGTTTTATAACTTACTACAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGTGTATTATATGACCGCGCAAGGCAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGGACGGCGAGAGCTCTTGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAGGGACTTGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGAGCGTCTTGCTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTGCCATTACAAAGTGAACGAAAAGAAGAAGTGTACGATAGGTTAATGTGGAAGTACGGGTATGAGG
AAGCAACCTCTCATCGTGCGAAGCAATCCGTTACAGAAATAAAGAGAAATTATCAGTCTGAAGAAGGCAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
GACGGCAGTCCATACCGTTATGCAACATGTAGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAACAAATTGCTG
GAATGGTAAATAAGGAATTGTTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTATCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAAGAAGATG
GTATTACGCTAATCGATTTCAAAACAGATACGATTGAAGGTAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGCTATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTGAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.328	100	0.536