Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FORC60_RS22165 Genome accession   NZ_CP020383
Coordinates   4180899..4181942 (-) Length   347 a.a.
NCBI ID   WP_001013167.1    Uniprot ID   -
Organism   Bacillus cereus strain FORC60     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4175899..4186942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC60_RS22120 (FORC60_4346) - 4176333..4176533 (-) 201 WP_000106083.1 YqzE family protein -
  FORC60_RS22125 (FORC60_4347) aroK 4176572..4177069 (-) 498 WP_000836624.1 shikimate kinase AroK -
  FORC60_RS22130 (FORC60_4348) - 4177185..4177835 (-) 651 WP_000183867.1 2OG-Fe(II) oxygenase -
  FORC60_RS22135 (FORC60_4349) comGG 4178010..4178380 (-) 371 Protein_4160 competence type IV pilus minor pilin ComGG -
  FORC60_RS22140 (FORC60_4350) comGF 4178377..4178847 (-) 471 WP_000923106.1 competence type IV pilus minor pilin ComGF -
  FORC60_RS22145 (FORC60_4351) - 4178817..4179119 (-) 303 WP_000829464.1 hypothetical protein -
  FORC60_RS22150 (FORC60_4352) comGD 4179112..4179567 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  FORC60_RS22155 (FORC60_4353) comGC 4179564..4179863 (-) 300 WP_001178684.1 competence type IV pilus major pilin ComGC -
  FORC60_RS22160 (FORC60_4354) comGB 4179875..4180912 (-) 1038 WP_044306401.1 competence type IV pilus assembly protein ComGB -
  FORC60_RS22165 (FORC60_4355) comGA 4180899..4181942 (-) 1044 WP_001013167.1 competence type IV pilus ATPase ComGA Machinery gene
  FORC60_RS22170 (FORC60_4356) - 4182148..4182843 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  FORC60_RS22175 (FORC60_4357) - 4182969..4183211 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  FORC60_RS22180 (FORC60_4358) - 4183319..4184713 (+) 1395 WP_001094344.1 L-cystine transporter -
  FORC60_RS22185 (FORC60_4359) - 4184792..4185184 (-) 393 WP_000875399.1 hypothetical protein -
  FORC60_RS22190 (FORC60_4360) - 4185272..4185487 (-) 216 WP_001008327.1 DUF3912 family protein -
  FORC60_RS22195 (FORC60_4361) - 4185740..4186051 (+) 312 WP_001093237.1 hypothetical protein -
  FORC60_RS22200 (FORC60_4362) - 4186086..4186574 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39092.47 Da        Isoelectric Point: 8.3536

>NTDB_id=222025 FORC60_RS22165 WP_001013167.1 4180899..4181942(-) (comGA) [Bacillus cereus strain FORC60]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTIQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFIDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=222025 FORC60_RS22165 WP_001013167.1 4180899..4181942(-) (comGA) [Bacillus cereus strain FORC60]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGATACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCCACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGTCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATAGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment