Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   BSK2_RS08640 Genome accession   NZ_CP020367
Coordinates   1659994..1660887 (+) Length   297 a.a.
NCBI ID   WP_014479775.1    Uniprot ID   A0AA96ZWR3
Organism   Bacillus subtilis strain GQJK2     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1654994..1665887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSK2_RS08620 (BSK2_08620) - 1655664..1657394 (+) 1731 WP_080529317.1 hypothetical protein -
  BSK2_RS08625 (BSK2_08625) ylqH 1657391..1657672 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  BSK2_RS08630 (BSK2_08630) sucC 1657845..1659002 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  BSK2_RS08635 (BSK2_08635) sucD 1659031..1659933 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  BSK2_RS08640 (BSK2_08640) dprA 1659994..1660887 (+) 894 WP_014479775.1 DNA-processing protein DprA Machinery gene
  BSK2_RS08645 (BSK2_08645) topA 1661075..1663150 (+) 2076 WP_003245599.1 type I DNA topoisomerase -
  BSK2_RS08650 (BSK2_08650) trmFO 1663226..1664533 (+) 1308 WP_003244725.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  BSK2_RS08655 (BSK2_08655) xerC 1664601..1665515 (+) 915 WP_080529318.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32907.21 Da        Isoelectric Point: 9.9142

>NTDB_id=221750 BSK2_RS08640 WP_014479775.1 1659994..1660887(+) (dprA) [Bacillus subtilis strain GQJK2]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQFYPKLPRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=221750 BSK2_RS08640 WP_014479775.1 1659994..1660887(+) (dprA) [Bacillus subtilis strain GQJK2]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCTGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAATTTTATCCAAAGCTCCCGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCTGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCACTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTATTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

99.663

100

0.997
  dprA Legionella pneumophila strain ERS1305867

38.462

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377

Multiple sequence alignment