Detailed information NTG0221619

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BLMD_RS06200
• Genome accession: NZ_CP020352
• Coordinates: 1238672..1242358 (+)
• Length: 1228 a.a.
• NCBI ID: WP_020450870.1
• Uniprot ID: -
• Organism: Bacillus paralicheniformis strain MDJK30
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1233672..1247358

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BLMD_RS06190 (BLMD_06190)	modB	1234419..1235075 (+)	657	WP_003180426.1	molybdate ABC transporter permease subunit	-
BLMD_RS06195 (BLMD_06195)	addB	1235185..1238685 (+)	3501	WP_020450869.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BLMD_RS06200 (BLMD_06200)	addA	1238672..1242358 (+)	3687	WP_020450870.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BLMD_RS06205 (BLMD_06205)	sbcD	1242424..1243605 (+)	1182	WP_003180431.1	exonuclease subunit SbcD	-
BLMD_RS06210 (BLMD_06210)	sbcC	1243602..1246994 (+)	3393	WP_020450871.1	exonuclease subunit SbcC	-
BLMD_RS06215 (BLMD_06215)	-	1247039..1247257 (-)	219	WP_016885923.1	spore germination protein	-

Sequence

Protein

• Length: 1228 a.a.
• Molecular weight: 141105.14 Da
• Isoelectric Point: 5.6938

>NTDB_id=221619 BLMD_RS06200 WP_020450870.1 1238672..1242358(+) (addA) [Bacillus paralicheniformis strain MDJK30]
MEISKPKGSNWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQEHPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPRGPAPRAENFLDDLAQIDRLIQQQNDFSALYELVPAVSFQRLKPCKGDAYDPRLVDEAADLRNSAKKQLEKLK
SDYFMRTPEQHLESLREMKPVIQTLVQLVLEYGKRFADAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDNGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPSNKATITELLLIENQSGAQDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDALLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPLVIDEIREHPARFQVSWLSAADLQAE
DIDQAGEERHGRLSQIQMGLPIDGSFEYEQQVRERLTWSYPYKDAAKVRTKQSVSEMKRQKEYEYGDRSLIRPTKDALLF
KRPSFMMAKGLTAAERGTAMHTVMQHIPLTRALEMNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLGAR
RVEREVPFNMTLSAKEVYPDLESADETVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQLY
ARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3687 bp

>NTDB_id=221619 BLMD_RS06200 WP_020450870.1 1238672..1242358(+) (addA) [Bacillus paralicheniformis strain MDJK30]
ATGGAGATTTCTAAACCGAAGGGCAGCAATTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACATC
CCGTAGATGTCGACAGGCTGCTTGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATTTGATCGATGTTGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTTGGCGATGAGGTGCTGGACGAGCTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCC
TTTTTTGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTCGTCAAGCGGGTCTACGAGTT
TTCCCGTTCGCATCCCGATCCGGAAGGCTGGCTTCACACATTTGTTGATCTGTATGACGCGGCTTCAGATACGAAAGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAGGGGATGCGGCAAAAGCTGACGCGTGCG
CTTGATTTGACAAAGCAGCCAAGGGGGCCTGCGCCGCGCGCCGAAAATTTCCTCGATGATTTAGCGCAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCGGCTCTGTACGAGCTTGTACCGGCCGTTTCGTTTCAAAGATTGAAGCCGTGCAAAG
GAGATGCGTACGATCCTCGATTGGTTGATGAGGCCGCAGATCTCCGCAACAGCGCGAAAAAGCAACTTGAAAAGCTGAAA
AGCGATTATTTTATGCGAACTCCCGAGCAGCACCTTGAGAGCTTGAGGGAAATGAAGCCGGTCATTCAGACGCTCGTCCA
GCTCGTGTTGGAGTATGGCAAGCGGTTTGCGGACGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGGGAAAAACCAAGCCGGCGAAGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCATGAAGTGCTTGTTGATGAATATCAGGATACGAACTTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCAGAACCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACAATGGCTCGGGTTCCGGTCAAAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAGCAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
GGCCGAATTGAAGCTTGGCGCGTCTTATCCGTCGAACAAAGCGACAATAACGGAGCTTCTGCTCATCGAAAACCAGAGCG
GCGCGCAGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGACAAATCCGCAGGCTG
ATAACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCACGGAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCGCAAATTATGGACGAGTTTAAACAGCAGGGGATCCCGGTATACGCGAACCTCTCAACGG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCTTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTAGGTCTTGATGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGATGAAAGCATACCTTGCTGCAGCAGATGGCGATGAAAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
CCCTGCTCAAAAAGTGGAGAGCGTATGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGACAGGCCAATTTGCGCGCGCTTTACGACAGGGCAAGATC
CTATGAAGCGACATCGTTCCGCGGTTTGTTCCGCTTTTTAAGGTTTATTGAGCGGATGCAGGAGCGGGGTGATGATCTCG
GAACGGCAAGAGCTCTGAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAGTCTTATCTGCTGGATAAAGAACTCGG
TTTCGGAACGAAGTTTATTCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTGATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCTCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCTAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCGGGGTCTGAGTGGCTGCTTCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCTGGTGATTGATGAGATCCGTGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCGGCCGCGGATCTTCAAGCAGAA
GATATTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCTCCCAAATTCAAATGGGGCTTCCGATCGATGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAAAGGCTGACATGGTCATATCCATACAAAGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGGCAGAAGGAGTATGAGTACGGCGACCGCTCGCTGATTCGCCCAACTAAGGATGCTCTCCTGTTT
AAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCGGCCGAACGAGGGACGGCGATGCACACCGTCATGCAGCATAT
TCCGCTGACCCGTGCGCTTGAAATGAATGAGCTCAGCCGCCTCCTAGACAGGCTTGTTGAGAAAGAGCTATTAACAGAAG
ACCAGCGGGCAGCGATTGAGGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGCGCCCGC
CGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCTGATCTTGAGTCAGCCGACGAAAC
TGTATTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCGACCGGA
TACACGGAAAGTACCGAAACGGATTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAGCTTTAT
GCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACATGTGCTCTT
ATTTTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1049-1220)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.73	100	0.719