Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BLMD_RS06195 Genome accession   NZ_CP020352
Coordinates   1235185..1238685 (+) Length   1166 a.a.
NCBI ID   WP_020450869.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain MDJK30     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1230185..1243685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLMD_RS06170 (BLMD_06170) - 1230854..1232221 (-) 1368 WP_025811704.1 sensor histidine kinase -
  BLMD_RS06175 (BLMD_06175) - 1232218..1232859 (-) 642 WP_043054576.1 response regulator transcription factor -
  BLMD_RS06180 (BLMD_06180) - 1232973..1233413 (-) 441 WP_020450867.1 ferritin-like domain-containing protein -
  BLMD_RS06185 (BLMD_06185) modA 1233652..1234410 (+) 759 WP_373368077.1 molybdate ABC transporter substrate-binding protein -
  BLMD_RS06190 (BLMD_06190) modB 1234419..1235075 (+) 657 WP_003180426.1 molybdate ABC transporter permease subunit -
  BLMD_RS06195 (BLMD_06195) addB 1235185..1238685 (+) 3501 WP_020450869.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BLMD_RS06200 (BLMD_06200) addA 1238672..1242358 (+) 3687 WP_020450870.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BLMD_RS06205 (BLMD_06205) sbcD 1242424..1243605 (+) 1182 WP_003180431.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134551.51 Da        Isoelectric Point: 5.4744

>NTDB_id=221618 BLMD_RS06195 WP_020450869.1 1235185..1238685(+) (addB) [Bacillus paralicheniformis strain MDJK30]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLQPESIRDMIEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYADEIRNAAVYVDGFHQFTPQEMNVLEQLMVHAEEITFSLTADQP
FTANSPNELHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHQQHSELRHLERYFDERPAKPYRGRTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEESGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDDDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDGEEKMLIHEPEQLDDEAQLEFLVNKG
AALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMEASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNMISAGLKKDGSLRSDSDVIAEEDFHVLRRHIRRTFQKAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDNVILERLKKEDETDGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=221618 BLMD_RS06195 WP_020450869.1 1235185..1238685(+) (addB) [Bacillus paralicheniformis strain MDJK30]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCCGATTTGGGCGGAACTATCCGCGCCCAAGTGTACAGTTTTTCAAGGCTTGCCTGGCGAGTGCTCCAGCATACCGGA
GGCATGAATCGGCCGTTTTTGACGGGGACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCATT
GTCTGCAGCCTGAAAGCATCCGGGACATGATAGAGGGGACGGGCAAATCGGAATACGAAGATGAACGGATCCTTTCCGAT
AAATTGCACGACTTATATATTTTGTACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAGGATTATTT
GACGCTTTTGGCCGAGCAGATTCCGTATGCGGATGAGATTAGAAACGCTGCCGTTTACGTGGACGGATTTCACCAATTTA
CGCCACAGGAGATGAACGTGCTGGAACAGCTGATGGTACATGCGGAGGAGATCACTTTTTCGCTGACGGCGGATCAGCCG
TTTACGGCCAACAGCCCGAATGAGCTCCATCTGTTCAGAATGACCGGGAAGGCGTACTATGACCTTTACCAGAAGGCGAA
GGAACTTGGCCTTGATCCGTCAGAAGTGCGGCTGGAAGAAACGAAACGGCACCAGCAACATTCGGAGCTCCGGCACCTTG
AACGCTATTTTGACGAGCGTCCTGCAAAACCGTATCGCGGACGGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATTCGCCAGGGCCGCTTCAGGCTTAGGGATATCGCCGT
TATTGCCCGCAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGTGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTCATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAACTGGCGGTAT
GAAGCGGTGTTCCGCTGTGTCAAAACGGAACTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCGTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGGT
ACGCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCACCGATCCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACCGTCAGGGAAATGGCCGAAGCCGTTTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTGGCCGAAGAAAGCGGTAGGATTGCAGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATA
TCGTTTGCTTTATTTCAGCAAATGATTGAAACCGGCTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCTCTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGCACATTTCTCATCGGTGTAAACGACGGTATAC
TTCCGGCGAGGCCTGCGGACGACGGCGTGCTTTCAGACGATGACCGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCT
GCGACGGGTCGAGAGCAGCTCTTGGATGAGAATTTTCTGATCTATATGACGCTTTCCAGCCCGTCTGAAAAGCTCTATGT
CTCCTATCCGATCGCAGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTAGTGAAACGGCTGAATGAGCTGTTCCCGG
ACGGCGAAGAGAAGATGCTGATTCATGAACCCGAGCAGCTGGACGACGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
GCCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATTTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAATATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACTTGAACCGGCATGTGAGCCGCGATTTATATGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAAGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGTTTAAAGCTGAAGGAACGTCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCGGCGCTGAAGCTGATTTCAGATCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAATGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTCGCGCCAAAGCTGCAAAAAGAGATTTTGCTCAGTTCGAAC
CGTCATCATTACGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGCGAGCATGCAAAAGCAAG
CGGGTTTGCCCCCGTCGGCATCGAGCTCGGATTCGGGGGGAAAGGTCCGCTTCCTCCAATGCGTTTTACCTTGAAGAACG
GCTGCACAATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTC
GACTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGCCTCGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACACATTCAACCGATTGGCTCGGCATGGAGGCATCCCCTGCAGGCGTGCTTTATTTTCATGTGCACGATC
CGATGATCCAGGCTTCAGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCTTGGGAGACCAGGAAGCGGTCAAGCTGATGGATACCACGCTGGAACAAGGCAGGTCGAACATGATCAGCGCAGGCTT
AAAAAAAGACGGCTCGCTCCGTTCGGACTCTGACGTTATTGCAGAAGAGGATTTTCATGTATTAAGGCGCCATATCCGCC
GGACATTTCAGAAGGCCGGCGAGGAGATCACAGACGGTAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCGTACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGGATATTAAAACCCGA
AAAAGACAACGTGATTTTGGAGCGGCTGAAAAAGGAGGATGAGACAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

73.82

99.914

0.738

Multiple sequence alignment