Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BLMD_RS02145 Genome accession   NZ_CP020352
Coordinates   391726..392172 (-) Length   148 a.a.
NCBI ID   WP_080131222.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain MDJK30     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 386726..397172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLMD_RS02120 (BLMD_02120) - 387341..388189 (-) 849 WP_196220020.1 transporter substrate-binding domain-containing protein -
  BLMD_RS02125 (BLMD_02125) - 388401..388952 (+) 552 WP_080131218.1 NAD(P)H-dependent oxidoreductase -
  BLMD_RS02130 (BLMD_02130) - 389038..389763 (+) 726 WP_080131219.1 AAA family ATPase -
  BLMD_RS02135 (BLMD_02135) - 389833..391269 (+) 1437 WP_080131220.1 glycoside hydrolase family 1 protein -
  BLMD_RS02140 (BLMD_02140) nin/comJ 391310..391708 (-) 399 WP_080131221.1 competence protein ComJ Regulator
  BLMD_RS02145 (BLMD_02145) nucA/comI 391726..392172 (-) 447 WP_080131222.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BLMD_RS02155 (BLMD_02155) - 392649..393452 (+) 804 WP_080131224.1 serine/threonine protein kinase -
  BLMD_RS02160 (BLMD_02160) - 393472..395175 (-) 1704 WP_080131225.1 methyl-accepting chemotaxis protein -
  BLMD_RS02165 (BLMD_02165) - 395293..396033 (-) 741 WP_080131226.1 ribonuclease YeeF family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16251.28 Da        Isoelectric Point: 5.7009

>NTDB_id=221609 BLMD_RS02145 WP_080131222.1 391726..392172(-) (nucA/comI) [Bacillus paralicheniformis strain MDJK30]
MDKIKSLLITLLAVAVVIIGIISGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIQKAIKKGHSKICTIDRDGAD
ERRKESLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKILFKIK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=221609 BLMD_RS02145 WP_080131222.1 391726..392172(-) (nucA/comI) [Bacillus paralicheniformis strain MDJK30]
ATGGACAAGATTAAATCTTTGCTGATCACCTTGCTCGCCGTCGCCGTCGTGATCATCGGCATTATATCCGGCGACTTTTT
CACTTCAGAACACCAGCCTTCAGGCCGGCAAAACGGCTCAAACTATGATGAAGTGCTGATTTTTCCGTCGGACCGCTACC
CTGAAACAGGCGCGCACATTCAAAAGGCCATCAAAAAAGGGCATTCCAAAATCTGCACGATCGACAGGGACGGAGCCGAC
GAACGCCGAAAGGAATCGTTAAAAGATGTTCCTTCCAAATCGGGCTATGACCGCGACGAGTGGCCGATGGCCATGTGCGA
AGAAGGCGGAACAGGTGCTTCCGTCGAGTATATCAGTCCTTCTGATAACCGTGGAGCAGGATCTTGGGTGGGCAATCAGG
TAAGCGATTATCCGGACGGCACGAAAATACTTTTCAAGATTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.946

99.324

0.635

Multiple sequence alignment