Detailed information NTG0220901

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: B5D85_RS01425
• Genome accession: NZ_CP020082
• Coordinates: 264684..265754 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain STAB120304
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 259684..270754

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5D85_RS01410 (B5D85_01410)	amiA	260212..262182 (+)	1971	WP_038433589.1	peptide ABC transporter substrate-binding protein	Regulator
B5D85_RS01415 (B5D85_01415)	amiC	262247..263749 (+)	1503	WP_011017334.1	ABC transporter permease	Regulator
B5D85_RS01420 (B5D85_01420)	amiD	263749..264675 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
B5D85_RS01425 (B5D85_01425)	amiE	264684..265754 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
B5D85_RS01430 (B5D85_01430)	amiF	265747..266670 (+)	924	WP_002986000.1	ATP-binding cassette domain-containing protein	Regulator
B5D85_RS10325 (B5D85_01435)	-	266708..266791 (-)	84	Protein_230	IS3 family transposase	-
B5D85_RS10030 (B5D85_01440)	-	266818..266997 (-)	180	WP_111695866.1	transposase	-
B5D85_RS01450 (B5D85_01450)	-	267293..268443 (-)	1151	Protein_232	IS3 family transposase	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=220901 B5D85_RS01425 WP_010921893.1 264684..265754(+) (amiE) [Streptococcus pyogenes strain STAB120304]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=220901 B5D85_RS01425 WP_010921893.1 264684..265754(+) (amiE) [Streptococcus pyogenes strain STAB120304]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATTGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537