Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GTHT12_RS12785 Genome accession   NZ_CP020030
Coordinates   2551612..2552682 (-) Length   356 a.a.
NCBI ID   WP_029760727.1    Uniprot ID   -
Organism   Geobacillus thermodenitrificans strain T12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2546612..2557682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTHT12_RS12745 (GTHT12_01933) - 2547445..2548239 (+) 795 WP_011887796.1 YqhG family protein -
  GTHT12_RS12750 (GTHT12_01934) - 2548316..2548495 (-) 180 WP_008879845.1 YqzE family protein -
  GTHT12_RS12755 (GTHT12_01935) comGG 2548541..2548930 (-) 390 WP_008879846.1 competence type IV pilus minor pilin ComGG -
  GTHT12_RS12760 (GTHT12_01936) comGF 2549064..2549549 (-) 486 WP_011887798.1 competence type IV pilus minor pilin ComGF -
  GTHT12_RS12765 (GTHT12_01937) - 2549486..2549812 (-) 327 WP_011887799.1 hypothetical protein -
  GTHT12_RS12770 (GTHT12_01938) comGD 2549796..2550233 (-) 438 WP_008879849.1 competence type IV pilus minor pilin ComGD -
  GTHT12_RS12775 (GTHT12_01939) comGC 2550223..2550519 (-) 297 WP_011887800.1 competence type IV pilus major pilin ComGC Machinery gene
  GTHT12_RS12780 (GTHT12_01940) comGB 2550587..2551615 (-) 1029 WP_011887801.1 competence type IV pilus assembly protein ComGB -
  GTHT12_RS12785 (GTHT12_01941) comGA 2551612..2552682 (-) 1071 WP_029760727.1 competence type IV pilus ATPase ComGA Machinery gene
  GTHT12_RS12790 (GTHT12_01942) - 2552882..2553580 (+) 699 WP_008879853.1 helix-turn-helix domain-containing protein -
  GTHT12_RS12795 (GTHT12_01943) - 2553744..2553986 (+) 243 WP_008879854.1 DUF2626 family protein -
  GTHT12_RS12800 (GTHT12_01944) - 2554017..2555144 (-) 1128 WP_087960149.1 SAM-dependent methyltransferase -
  GTHT12_RS12805 (GTHT12_01945) - 2555376..2556008 (-) 633 WP_008879856.1 MBL fold metallo-hydrolase -
  GTHT12_RS12810 (GTHT12_01946) - 2556188..2556364 (+) 177 WP_008879857.1 DUF2759 domain-containing protein -
  GTHT12_RS12815 (GTHT12_01947) - 2556400..2557563 (-) 1164 WP_008879858.1 M14 family metallocarboxypeptidase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39595.73 Da        Isoelectric Point: 8.6314

>NTDB_id=220533 GTHT12_RS12785 WP_029760727.1 2551612..2552682(-) (comGA) [Geobacillus thermodenitrificans strain T12]
MDDIEQVANRLLAEAVQRRASDLHLVPRRHDAIIRLRLDGMLIDVGTISKETAERVIVHFKFLAGMDIGERRRPQSGAME
VNESGETVYLRLSTLPTLYDESLVIRLLPQRFSLPLRELSLFSHSTARLFSFMQQPQGLVLLTGPTGSGKTTTLYTLLDV
CQAERQRNIITLEDPIEKRNDRFLQVQINEKAGITYAASLKAALRHDPDVLMVGEIRDHDTAAIAVRSALTGHLVVSTMH
AADAVGAVYRLHEFGVSPGDLAETLLAVSAQRLVELRCPLCGDNCHPACRRLQRRRRAAVHELLYGSALFDVIRSLSGEG
EQRLRPHLTLARLIRKGIALGYLPTRALELVGGGER

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=220533 GTHT12_RS12785 WP_029760727.1 2551612..2552682(-) (comGA) [Geobacillus thermodenitrificans strain T12]
CTGGATGATATCGAACAAGTAGCGAACCGTCTCCTCGCCGAAGCGGTGCAACGCCGCGCCTCTGACCTTCATCTCGTCCC
GCGCCGACATGATGCGATCATTCGCCTCCGCCTTGACGGCATGCTCATCGATGTTGGTACAATCTCGAAAGAAACCGCTG
AACGCGTCATCGTCCATTTCAAATTTTTGGCTGGCATGGATATTGGCGAACGCCGTCGTCCGCAAAGCGGCGCGATGGAA
GTGAATGAGTCCGGGGAAACGGTGTATTTGCGCTTATCCACGCTGCCGACGCTTTATGATGAAAGCCTCGTCATCCGCCT
TCTACCGCAGCGCTTTTCGCTGCCGCTTCGTGAACTATCCTTATTTTCCCACTCCACCGCACGATTGTTTTCCTTTATGC
AGCAGCCACAAGGACTCGTGTTGCTGACCGGCCCAACCGGGTCGGGAAAGACGACGACATTGTATACGCTTCTCGACGTT
TGCCAAGCGGAAAGACAACGTAACATTATCACGTTGGAAGATCCGATTGAAAAGCGGAATGATCGGTTTTTGCAAGTGCA
GATCAATGAAAAAGCAGGTATCACGTATGCAGCCAGCTTGAAAGCCGCTTTGCGCCATGACCCGGATGTGTTAATGGTCG
GCGAGATCCGCGACCATGATACGGCAGCGATCGCCGTACGATCAGCGTTGACAGGGCACTTGGTGGTATCGACCATGCAT
GCTGCTGATGCAGTCGGCGCAGTGTATCGGCTCCATGAGTTCGGGGTGTCTCCCGGCGACTTGGCTGAGACGTTGCTTGC
TGTTTCGGCGCAGCGTCTTGTTGAGCTTCGCTGCCCGCTATGCGGTGACAATTGCCATCCGGCTTGCCGTCGGCTTCAAC
GAAGACGGCGTGCTGCCGTTCATGAGTTGCTTTACGGCTCAGCGCTCTTTGATGTCATCCGTTCGCTGTCGGGGGAAGGC
GAGCAACGGCTCCGCCCGCATCTGACATTAGCACGTCTTATCCGCAAAGGAATCGCGCTCGGGTATTTGCCGACTCGCGC
GCTTGAACTTGTCGGAGGGGGAGAACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

51.585

97.472

0.503
  pilB Glaesserella parasuis strain SC1401

37.83

95.787

0.362

Multiple sequence alignment