Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   BV121_RS01945 Genome accession   NZ_CP020008
Coordinates   394897..395436 (+) Length   179 a.a.
NCBI ID   WP_042611895.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 5P28H1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389897..400436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV121_RS01925 (BV121_392) - 390353..391273 (-) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -
  BV121_RS01930 (BV121_393) - 391273..392154 (-) 882 WP_105182547.1 metal ABC transporter substrate-binding protein -
  BV121_RS01935 (BV121_394) - 392520..393398 (-) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  BV121_RS01940 (BV121_396) - 393645..394796 (+) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  BV121_RS01945 (BV121_397) pilF/pilF2 394897..395436 (+) 540 WP_042611895.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BV121_RS01950 (BV121_398) - 395508..396419 (+) 912 WP_042611894.1 helix-turn-helix domain-containing protein -
  BV121_RS01955 (BV121_399) ispG 396428..397534 (+) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BV121_RS01960 (BV121_400) hisS 397544..398815 (+) 1272 WP_038440737.1 histidine--tRNA ligase -
  BV121_RS01965 (BV121_401) - 398833..399447 (+) 615 WP_011272010.1 YfgM family protein -
  BV121_RS01970 (BV121_402) iscX 399498..399692 (-) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  BV121_RS01975 (BV121_403) fdx 399692..400033 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20598.25 Da        Isoelectric Point: 8.6216

>NTDB_id=219830 BV121_RS01945 WP_042611895.1 394897..395436(+) (pilF/pilF2) [Haemophilus influenzae strain 5P28H1]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSTLAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=219830 BV121_RS01945 WP_042611895.1 394897..395436(+) (pilF/pilF2) [Haemophilus influenzae strain 5P28H1]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAACACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment