Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   BV085_RS02340 Genome accession   NZ_CP020007
Coordinates   481324..481863 (+) Length   179 a.a.
NCBI ID   WP_005686810.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 48P153H1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476324..486863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV085_RS02320 (BV085_475) - 476781..477701 (-) 921 WP_005686805.1 ATP-binding cassette domain-containing protein -
  BV085_RS02325 (BV085_476) - 477701..478582 (-) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  BV085_RS02330 (BV085_477) - 478947..479825 (-) 879 WP_005686807.1 D-alanyl-D-alanine carboxypeptidase family protein -
  BV085_RS02335 (BV085_479) - 480072..481223 (+) 1152 WP_005686808.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  BV085_RS02340 (BV085_480) pilF/pilF2 481324..481863 (+) 540 WP_005686810.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BV085_RS02345 (BV085_481) - 481935..482846 (+) 912 WP_005686812.1 helix-turn-helix domain-containing protein -
  BV085_RS02350 (BV085_482) ispG 482855..483961 (+) 1107 WP_005686814.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BV085_RS02355 (BV085_483) hisS 483971..485242 (+) 1272 WP_005686816.1 histidine--tRNA ligase -
  BV085_RS02360 (BV085_484) - 485260..485874 (+) 615 WP_005686818.1 YfgM family protein -
  BV085_RS02365 (BV085_485) iscX 485925..486119 (-) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  BV085_RS02370 (BV085_486) fdx 486119..486460 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20595.25 Da        Isoelectric Point: 8.6216

>NTDB_id=219794 BV085_RS02340 WP_005686810.1 481324..481863(+) (pilF/pilF2) [Haemophilus influenzae strain 48P153H1]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=219794 BV085_RS02340 WP_005686810.1 481324..481863(+) (pilF/pilF2) [Haemophilus influenzae strain 48P153H1]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTAGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment