Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   B2D37_RS14955 Genome accession   NZ_CP019947
Coordinates   3289660..3291879 (+) Length   739 a.a.
NCBI ID   WP_079203430.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3284660..3296879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS14935 - 3285638..3286906 (-) 1269 WP_079203427.1 malic enzyme-like NAD(P)-binding protein -
  B2D37_RS14940 - 3287011..3288453 (-) 1443 WP_079203428.1 M48 family metalloprotease -
  B2D37_RS14945 - 3288450..3289250 (-) 801 WP_079204995.1 thermonuclease family protein -
  B2D37_RS14950 rpmE 3289277..3289492 (-) 216 WP_079203429.1 50S ribosomal protein L31 -
  B2D37_RS14955 priA 3289660..3291879 (+) 2220 WP_079203430.1 primosomal protein N' Machinery gene
  B2D37_RS14960 argS 3292072..3293835 (+) 1764 WP_079203431.1 arginine--tRNA ligase -
  B2D37_RS14965 - 3293836..3294522 (+) 687 WP_079203432.1 SPOR domain-containing protein -
  B2D37_RS14970 hslV 3294719..3295252 (+) 534 WP_079204996.1 ATP-dependent protease subunit HslV -
  B2D37_RS14975 hslU 3295280..3296620 (+) 1341 WP_079203433.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82046.34 Da        Isoelectric Point: 8.5086

>NTDB_id=219091 B2D37_RS14955 WP_079203430.1 3289660..3291879(+) (priA) [Pseudomonas sp. CC6-YY-74]
MPDAILRIALPSPLRRLFDYRAPPGVPRSALQPGARLRVPFGRRELIGILIEVTDHSEVPTGKLKPALQLLDAQSPLPPA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEVRQERFWHLVDGAQLDDPRLSRAPRQREALQTIAQHPHGVAHQLL
GQLQLNKESLGQLREKGLVRVEIRRHAASERHLNWLAEPELPLNPEQRAAAEAVRTGFGHFNAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFERRFNARIALLHSGVNDRERLDAWLAARDGEADIIIGTRSALFTPMQRPGL
IIIDEEHDASYKQQEGLRYHARDLAVVRAHQENIPILLGSATPSLESLHNAHSGRYAMLHLHQRAGGAQQPRFLRLDVKS
RPLDSGISGPMQQAIAKTLATGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRSGELRCHHCGHVERQPSNC
PKCNNVDLRPVGAGTERAEERLGILFPQVPVLRVDRDSTSRKEAMTSLFNTVQRGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQLIVQVAGRAGRAEEPGKVIIQSHLADHPLLVQLTEQGYFAFAEQALSERRSAGLPPFCHL
ALLRAEAHKPGQAEGFLDEACSEAELLVSELKLSGIELLGPVPAPMERRAGRYRAQLLVQANARAPLHRLLSTWMLALEQ
MPSGRAVRWSLDVDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=219091 B2D37_RS14955 WP_079203430.1 3289660..3291879(+) (priA) [Pseudomonas sp. CC6-YY-74]
GTGCCCGACGCCATTCTGCGCATTGCTCTGCCCTCACCGCTGCGCCGCCTGTTCGACTACCGTGCGCCGCCCGGCGTACC
GCGCAGCGCCCTGCAGCCGGGCGCACGATTGCGCGTGCCCTTTGGCCGGCGCGAGCTGATCGGCATCCTGATCGAGGTGA
CGGATCACAGTGAGGTCCCCACAGGCAAGCTCAAACCCGCCCTGCAACTGCTCGATGCCCAGTCGCCGCTGCCGCCAGCG
CTGTTCAAGCTGTGCCTGTGGACGGCGCAGTATTACCAGCACAGCCTGGGCGACACCCTCAGCTGGGCGTTGCCGGTATT
GCTGCGCCAGGGCGAACCGGCGGAGGTTCGCCAGGAGCGCTTCTGGCACCTGGTCGACGGCGCCCAACTGGATGACCCGC
GCCTGAGCCGCGCACCGCGCCAGCGCGAGGCCCTGCAAACCATCGCCCAGCACCCGCACGGCGTTGCTCATCAACTGCTT
GGCCAGTTGCAACTGAATAAGGAAAGCCTGGGTCAGCTGCGTGAGAAAGGCCTGGTGCGCGTCGAGATACGCCGGCATGC
CGCCAGTGAACGCCACCTCAACTGGCTGGCGGAACCGGAACTGCCCCTGAACCCGGAGCAACGCGCGGCAGCCGAAGCGG
TTCGCACCGGCTTCGGCCACTTCAATGCCTTCCTGCTCGCTGGGGTCACCGGTAGCGGCAAGACCGAGGTCTATCTGCAG
CTGATCCGCGAGACCCTGGAAGCCGGCAAGCAGGCCCTGGTACTAATCCCGGAGATCAACCTCGGCCCACAGACCCTGGC
GCGCTTCGAGCGGCGCTTCAACGCGCGAATCGCCCTGCTCCACTCCGGGGTCAACGACCGCGAGCGCCTGGACGCCTGGC
TGGCCGCCCGCGACGGCGAGGCCGACATCATCATCGGCACCCGCTCGGCACTGTTCACGCCAATGCAGCGCCCCGGCCTG
ATCATCATCGACGAGGAACATGACGCGTCCTACAAGCAGCAGGAAGGCCTGCGCTACCACGCCCGCGACCTGGCCGTGGT
GCGCGCGCACCAGGAAAACATTCCGATCCTGCTCGGCTCGGCCACGCCTTCCCTGGAAAGCCTGCACAACGCCCACAGCG
GCCGTTACGCCATGCTGCACCTGCACCAGCGCGCCGGCGGCGCCCAACAACCGCGCTTTCTGCGTCTGGACGTGAAGAGC
CGGCCGCTCGACTCCGGCATTTCCGGCCCCATGCAGCAAGCCATCGCCAAGACCCTGGCGACCGGCCAGCAGGTGCTGGT
GTTTCTCAACCGCCGCGGCTTCGCCCCGACCCTGCTCTGCCACGACTGCGGCTGGCTGTCGGAATGCCCACGCTGCGATG
CGCGGATGACCGTGCACCAGCGCTCCGGCGAGTTGCGCTGCCATCACTGCGGGCACGTCGAGCGCCAGCCGAGCAACTGC
CCGAAGTGCAACAACGTCGACCTGCGCCCGGTCGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGGCATTCTCTTTCC
GCAGGTGCCGGTATTGCGCGTGGACCGCGACAGCACCTCGCGCAAGGAGGCCATGACCAGCCTGTTCAACACCGTGCAAC
GCGGCGAACCGTGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGCCACCATTTCCCCCGCGTGACCCTGGTGGCGATT
CTGGATGCCGACGGCGGGCTGTTCTCCGCCGATTTTCGCGCCAGCGAGCGCATGGCCCAGCTGATCGTCCAGGTCGCCGG
CCGCGCCGGCCGCGCCGAGGAACCCGGCAAAGTAATCATTCAGAGTCACCTGGCGGACCACCCATTATTGGTGCAACTGA
CCGAACAGGGCTACTTCGCCTTCGCCGAGCAAGCCTTAAGCGAACGCCGCAGCGCCGGCTTGCCGCCGTTCTGCCACCTG
GCTCTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAAGGTTTTCTCGATGAGGCCTGCAGCGAAGCCGAACTGCT
GGTGAGCGAACTCAAGCTGAGCGGCATCGAGCTGCTTGGCCCAGTTCCGGCACCGATGGAACGCCGCGCCGGCCGTTACC
GCGCGCAACTCTTGGTGCAAGCCAACGCCCGCGCGCCGCTGCACCGCCTGCTCAGCACCTGGATGCTGGCGCTGGAGCAA
ATGCCCAGCGGCCGCGCGGTCCGTTGGTCGCTGGATGTCGACCCGATAGATTTGTTTTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(520-592)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-105)

DEAD/DEAH box helicase (DEAD)

(210-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment