Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CWI21_RS28195 Genome accession   NZ_CP025055
Coordinates   6074389..6075036 (-) Length   215 a.a.
NCBI ID   WP_237047402.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PB350     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6069389..6080036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI21_RS28175 (CWI21_28800) aroB 6070188..6071294 (-) 1107 WP_003095831.1 3-dehydroquinate synthase -
  CWI21_RS28180 (CWI21_28805) aroK 6071344..6071862 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CWI21_RS28185 (CWI21_28810) pilQ 6071874..6074018 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  CWI21_RS28190 (CWI21_28815) pilP 6074072..6074458 (-) 387 Protein_5586 type 4a pilus biogenesis lipoprotein PilP -
  CWI21_RS28195 (CWI21_28820) pilO 6074389..6075036 (-) 648 WP_237047402.1 type 4a pilus biogenesis protein PilO Machinery gene
  CWI21_RS28200 (CWI21_28825) pilN 6075033..6075629 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  CWI21_RS28205 (CWI21_28830) pilM 6075629..6076693 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  CWI21_RS28210 (CWI21_28835) - 6076878..6079349 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23623.05 Da        Isoelectric Point: 5.3469

>NTDB_id=217126 CWI21_RS28195 WP_237047402.1 6074389..6075036(-) (pilO) [Pseudomonas aeruginosa strain PB350]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSISSSRTRPSPIAPRRYAARSSRR

Nucleotide


Download         Length: 648 bp        

>NTDB_id=217126 CWI21_RS28195 WP_237047402.1 6074389..6075036(-) (pilO) [Pseudomonas aeruginosa strain PB350]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCAGTTCCAGCCGTACGAGGCCTTCACCTATAGCGCCGCGTCGCTACGCAGCCCGTTCCAGCC
GCCGGTGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

44.271

89.302

0.395
  pilO Acinetobacter baumannii D1279779

41.799

87.907

0.367