Detailed information NTG0021586

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NMB_RS01745
• Genome accession: NC_003112
• Coordinates: 339992..340852 (-)
• Length: 286 a.a.
• NCBI ID: WP_002212335.1
• Uniprot ID: Q9K154
• Organism: Neisseria meningitidis MC58
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 334992..345852

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMB_RS01705 (NMB0325)	rplU	334996..335304 (-)	309	WP_002216394.1	50S ribosomal protein L21	-
NMB_RS01715 (NMB0326)	ispB	335528..336502 (+)	975	WP_002216396.1	octaprenyl diphosphate synthase	-
NMB_RS01720 (NMB0327)	-	336507..336954 (+)	448	Protein_322	DUF441 domain-containing protein	-
NMB_RS01730 (NMB0329)	pilF	337395..339071 (+)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NMB_RS01735 (NMB0330)	-	339156..339365 (-)	210	WP_002224872.1	DNA gyrase inhibitor YacG	-
NMB_RS01740 (NMB0331)	coaE	339358..339990 (-)	633	WP_002224873.1	dephospho-CoA kinase	-
NMB_RS01745 (NMB0332)	pilD	339992..340852 (-)	861	WP_002212335.1	prepilin peptidase	Machinery gene
NMB_RS01750 (NMB0333)	pilG	340926..342158 (-)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
NMB_RS01755 (NMB0334)	pgi	342515..344158 (+)	1644	WP_002224874.1	glucose-6-phosphate isomerase	-
NMB_RS01760	-	344128..344358 (+)	231	WP_009348191.1	hypothetical protein	-
NMB_RS01765 (NMB0335)	dapD	344368..345189 (+)	822	WP_002224875.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31305.31 Da
• Isoelectric Point: 8.4690

>NTDB_id=21586 NMB_RS01745 WP_002212335.1 339992..340852(-) (pilD) [Neisseria meningitidis MC58]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=21586 NMB_RS01745 WP_002212335.1 339992..340852(-) (pilD) [Neisseria meningitidis MC58]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGAAGTTTCTTAAA
TGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGAT
TGGTACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTAATTTGGCTGGGTCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTCGCCGG
CTATGGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTACTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

3D structure

• AlphaFold DB: Q9K154

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	97.544	99.65	0.972
pilD	Vibrio cholerae strain A1552	45.833	100	0.462
pilD	Vibrio campbellii strain DS40M4	46.377	96.503	0.448
pilD	Acinetobacter baumannii D1279779	48.106	92.308	0.444
pilD	Acinetobacter nosocomialis M2	48.106	92.308	0.444