Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BRW84_RS03200 Genome accession   NZ_CP019430
Coordinates   684921..686393 (-) Length   490 a.a.
NCBI ID   WP_005881476.1    Uniprot ID   C3XAZ7
Organism   Oxalobacter formigenes OXCC13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 679921..691393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRW84_RS11680 (BRW84_03185) - 680531..683158 (-) 2628 WP_005881482.1 hypothetical protein -
  BRW84_RS03190 (BRW84_03195) - 683915..684274 (-) 360 WP_005881480.1 DsrE family protein -
  BRW84_RS03195 (BRW84_03200) hpf 684503..684853 (-) 351 WP_005881478.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BRW84_RS03200 (BRW84_03205) rpoN/rpoN1 684921..686393 (-) 1473 WP_005881476.1 RNA polymerase factor sigma-54 Machinery gene
  BRW84_RS03205 (BRW84_03210) lptB 686408..687151 (-) 744 WP_005881474.1 LPS export ABC transporter ATP-binding protein -
  BRW84_RS03210 (BRW84_03215) lptA 687151..687705 (-) 555 WP_005881472.1 lipopolysaccharide transport periplasmic protein LptA -
  BRW84_RS03215 (BRW84_03220) lptC 687727..688296 (-) 570 WP_231284505.1 LPS export ABC transporter periplasmic protein LptC -
  BRW84_RS03220 (BRW84_03225) - 688329..688859 (-) 531 WP_005881469.1 KdsC family phosphatase -
  BRW84_RS03225 (BRW84_03230) - 688856..689872 (-) 1017 WP_005881466.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 490 a.a.        Molecular weight: 55446.07 Da        Isoelectric Point: 5.7266

>NTDB_id=215712 BRW84_RS03200 WP_005881476.1 684921..686393(-) (rpoN/rpoN1) [Oxalobacter formigenes OXCC13]
MKQKLQTSFSQHLALTPQLQQSIRLLQLSSQELNQELEQALQENPLLERVDDPLATSTRLSGDGSVISTTKQPETASHTD
SGDFSGERTGDVSSNEEHTSDSERMDSLWNVPVPSERQPDDERSKPQIEAPPESLREHLISQMRLSVISQRQRALVELII
DALDANGFLQESLPEMLEWLPQELDISLKELEEALSCVQDFEPAGVGARTSAECLAIQIRLLPRIPYVVRKRAMNIVENH
LELFARHDFVRLKKVLECDDEDLKEAQEVIHRCNPHPGAIYATDTAETVIPEIIVSKKENCWVAELNPDAMPKIRVNQLY
ANILRESGSKAALSVQLREAKWLIRNINQRYETILKTSQAIVERQQNFFSFGATSMRPLVLREIADTLGLHESTISRVSN
QKFMRTPHGILELKYFFGSSVATETGGEASSTAIREKIRQLIETEDKKKPLSDNRIAQLLERQGLVVARRTVAKYRDILK
IPPASLRKKL

Nucleotide


Download         Length: 1473 bp        

>NTDB_id=215712 BRW84_RS03200 WP_005881476.1 684921..686393(-) (rpoN/rpoN1) [Oxalobacter formigenes OXCC13]
ATGAAACAAAAACTTCAAACCAGCTTTAGCCAACATCTAGCCTTAACCCCGCAATTACAGCAGTCCATCCGTCTTCTGCA
ACTGTCGAGCCAGGAACTGAATCAGGAACTGGAACAGGCCTTGCAGGAAAATCCTCTTCTGGAAAGAGTGGATGATCCTT
TAGCCACTTCAACGCGCCTGTCGGGGGATGGTTCTGTCATATCGACGACAAAACAGCCGGAAACAGCCAGCCATACCGAT
TCCGGAGATTTTTCCGGCGAAAGAACGGGTGACGTTTCCAGCAATGAAGAACATACCTCCGATTCAGAACGGATGGACAG
TCTCTGGAATGTCCCGGTACCATCCGAGCGTCAACCGGACGACGAACGTTCCAAACCCCAAATCGAAGCACCCCCTGAGT
CCTTGCGCGAACATTTGATTTCGCAAATGAGACTGTCCGTCATCAGCCAGCGCCAGCGTGCATTGGTTGAATTGATCATC
GATGCCCTGGATGCCAATGGTTTCCTGCAGGAGAGCTTGCCTGAAATGCTCGAATGGCTCCCTCAAGAACTCGATATCAG
CCTAAAAGAACTGGAAGAAGCCCTCTCCTGCGTACAGGATTTCGAACCTGCCGGGGTAGGTGCCAGAACGAGTGCTGAAT
GCCTCGCCATCCAGATCCGTCTGCTGCCCCGTATCCCGTATGTCGTTCGGAAACGGGCCATGAATATCGTTGAAAACCAC
CTCGAGCTGTTTGCACGACACGATTTTGTCCGTTTGAAAAAAGTTCTGGAATGTGACGATGAAGACCTGAAGGAAGCTCA
GGAAGTCATTCACCGATGCAATCCACATCCTGGGGCGATATATGCTACCGACACGGCTGAAACGGTCATTCCTGAAATTA
TCGTCAGCAAAAAGGAAAATTGCTGGGTTGCCGAGCTGAATCCTGACGCCATGCCGAAAATACGGGTCAATCAGTTGTAT
GCAAATATTTTGAGAGAATCGGGCAGCAAGGCAGCGCTTTCAGTTCAGCTTCGGGAGGCTAAATGGTTGATCAGGAATAT
AAATCAGCGATATGAAACCATACTCAAAACCAGTCAGGCCATCGTGGAGCGCCAGCAGAATTTCTTCTCTTTTGGGGCAA
CATCAATGCGCCCTCTTGTTTTGCGTGAAATAGCTGATACACTAGGACTACACGAGAGCACAATATCCCGTGTAAGCAAC
CAGAAATTCATGCGAACCCCGCATGGCATTTTAGAGTTGAAATACTTTTTTGGAAGTTCGGTCGCAACGGAAACTGGTGG
GGAAGCGTCTTCTACAGCCATTCGGGAAAAAATCAGGCAACTGATTGAAACCGAAGACAAGAAAAAGCCACTTTCTGACA
ACAGGATTGCACAGCTTCTGGAAAGACAGGGTCTGGTCGTGGCAAGAAGAACGGTGGCAAAATATCGCGACATTCTGAAA
ATACCTCCAGCTTCCCTGAGAAAAAAATTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-489)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C3XAZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.581

100

0.533

Multiple sequence alignment