Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BWP24_RS01510 Genome accession   NZ_CP019293
Coordinates   312383..313399 (+) Length   338 a.a.
NCBI ID   WP_005432712.1    Uniprot ID   -
Organism   Vibrio campbellii strain LMB29     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 307383..318399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWP24_RS01495 (BWP24_01535) - 307748..308476 (-) 729 WP_077200153.1 redoxin family protein -
  BWP24_RS01500 (BWP24_01540) oxyR 308651..309559 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  BWP24_RS01505 (BWP24_01545) - 309637..312204 (-) 2568 WP_047482703.1 penicillin-binding protein 1A -
  BWP24_RS01510 (BWP24_01550) pilM 312383..313399 (+) 1017 WP_005432712.1 type IV pilus biogenesis protein PilM Machinery gene
  BWP24_RS01515 (BWP24_01555) pilN 313383..313958 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  BWP24_RS01520 (BWP24_01560) pilO 313951..314544 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  BWP24_RS01525 (BWP24_01565) pilP 314534..315049 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  BWP24_RS01530 (BWP24_01570) pilQ 315084..316826 (+) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  BWP24_RS01535 (BWP24_01575) aroK 317015..317533 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37860.29 Da        Isoelectric Point: 6.3141

>NTDB_id=214624 BWP24_RS01510 WP_005432712.1 312383..313399(+) (pilM) [Vibrio campbellii strain LMB29]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=214624 BWP24_RS01510 WP_005432712.1 312383..313399(+) (pilM) [Vibrio campbellii strain LMB29]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGGGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCTGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACCCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACCGAACAAGGTGAATTATTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAGCAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTCTCTAAGCGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

98.225

100

0.982
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment