Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CS058_RS12250 Genome accession   NZ_CP024477
Coordinates   2509208..2510935 (+) Length   575 a.a.
NCBI ID   WP_052151401.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 12939     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2504208..2515935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS058_RS12230 - 2505264..2506016 (+) 753 WP_003110095.1 class I SAM-dependent methyltransferase -
  CS058_RS12235 - 2506040..2506831 (-) 792 WP_034026307.1 alpha/beta fold hydrolase -
  CS058_RS12240 - 2507009..2507971 (+) 963 WP_237754637.1 GlxA family transcriptional regulator -
  CS058_RS12245 - 2508415..2509218 (+) 804 WP_034026309.1 ABC transporter permease -
  CS058_RS12250 pilF 2509208..2510935 (+) 1728 WP_052151401.1 GspE/PulE family protein Machinery gene
  CS058_RS12255 - 2510940..2512127 (+) 1188 WP_003090520.1 type II secretion system F family protein -
  CS058_RS12260 gspG 2512181..2512615 (+) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  CS058_RS12265 - 2512584..2512994 (+) 411 WP_003110088.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CS058_RS12270 - 2512994..2513419 (+) 426 WP_003110087.1 PulJ/GspJ family protein -
  CS058_RS12275 - 2513416..2514006 (+) 591 WP_016852732.1 PulJ/GspJ family protein -
  CS058_RS12280 - 2513996..2515075 (+) 1080 WP_034026310.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63654.73 Da        Isoelectric Point: 5.2400

>NTDB_id=212947 CS058_RS12250 WP_052151401.1 2509208..2510935(+) (pilF) [Pseudomonas aeruginosa strain 12939]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPMVGADL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLQRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLNFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=212947 CS058_RS12250 WP_052151401.1 2509208..2510935(+) (pilF) [Pseudomonas aeruginosa strain 12939]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCTGAGTGGATACCGCTCGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGATGGTGGGGGCCGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGAAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAATGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGTGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCAGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGAACTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGTGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.519

98.435

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.448

98.609

0.379
  pilF Neisseria gonorrhoeae MS11

36.667

99.13

0.363

Multiple sequence alignment