Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   COG33_RS12475 Genome accession   NZ_CP024240
Coordinates   2346012..2346671 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O114:H49 strain 90-9280     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2341012..2351671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COG33_RS12450 plsC 2341159..2341896 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  COG33_RS12455 parC 2342129..2344387 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  COG33_RS12465 ygiV 2344933..2345415 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  COG33_RS12470 ygiW 2345468..2345860 (-) 393 WP_001356032.1 OB fold stress tolerance protein YgiW -
  COG33_RS12475 ciaR 2346012..2346671 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  COG33_RS12480 qseC 2346668..2348017 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  COG33_RS12485 ygiZ 2348063..2348395 (-) 333 WP_000912120.1 DUF2645 family protein -
  COG33_RS12490 mdaB 2348714..2349295 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  COG33_RS12495 ygiN 2349326..2349640 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  COG33_RS12500 parE 2349688..2351580 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=211785 COG33_RS12475 WP_001221502.1 2346012..2346671(+) (ciaR) [Escherichia coli O114:H49 strain 90-9280]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=211785 COG33_RS12475 WP_001221502.1 2346012..2346671(+) (ciaR) [Escherichia coli O114:H49 strain 90-9280]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment