Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   XF_RS06410 Genome accession   NC_002488
Coordinates   1469588..1471288 (+) Length   566 a.a.
NCBI ID   WP_031336720.1    Uniprot ID   -
Organism   Xylella fastidiosa 9a5c     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1464588..1476288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XF_RS06400 (XF1514) - 1464666..1465577 (+) 912 WP_010894015.1 AEC family transporter -
  XF_RS06405 (XF1516) - 1466282..1469296 (+) 3015 WP_010894017.1 YadA-like family protein -
  XF_RS06410 (XF1517) pilF 1469588..1471288 (+) 1701 WP_031336720.1 type II secretion system ATPase GspE Machinery gene
  XF_RS06415 (XF1518) - 1471324..1472541 (+) 1218 WP_010894019.1 type II secretion system protein XpsF -
  XF_RS06420 (XF1519) gspG 1472758..1473192 (+) 435 WP_004083686.1 type II secretion system major pseudopilin GspG -
  XF_RS06425 (XF1520) - 1473198..1473704 (+) 507 WP_010894021.1 type II secretion system protein XpsH -
  XF_RS06430 (XF1521) - 1473701..1474099 (+) 399 WP_010894022.1 type II secretion system protein XpsI -
  XF_RS06435 (XF1522) - 1474096..1474722 (+) 627 WP_010894023.1 type II secretion system protein J -
  XF_RS06440 (XF1523) - 1474722..1475567 (+) 846 WP_010894024.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62610.39 Da        Isoelectric Point: 5.0203

>NTDB_id=21124 XF_RS06410 WP_031336720.1 1469588..1471288(+) (pilF) [Xylella fastidiosa 9a5c]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPELSA
EVQGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGATLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFIDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPTHAERYLASPEQVARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDALRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=21124 XF_RS06410 WP_031336720.1 1469588..1471288(+) (pilF) [Xylella fastidiosa 9a5c]
ATGAATGTAAGTATGGAAGCATCCATCGTCGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGCGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGCTGGGTCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTGTCGGCC
GAGGTGCAAGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGTCCGCTTGCTGAACATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGCTGCGCCTTGCCACAGGGGCAACATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAGGGCCGCAGCGTCATGGGGATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATCCAGCGTGCAGTTGAATTACGTGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAA
ATTATGGCCAAACTTAATATTGCTGAACGTCGCTTGCCTCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGATCGTGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTATTGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTG
CTGGTCACTGGCCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATCGAAGGTATCAATCAGATCCAGGCTAAGCCGCAGATCGGGCTGG
ATTTTGCCAGTGCATTACGCAGCATTGTGCGTCAGGATCCAGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACAGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTTGATATGGGTGTGGAAGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTACGCATGCTGAGCGTTATCTTGCCTCGCCAGAGCAGGTTGCGCGTTTTGATTTGCGCCGTTTACAG
CCGGAGGGTGATATTTATTTGTATCGTCCGCGCGCTTCAGCGTTGGCACCCACTGGGTATCTGGGTCGTACGGCGATCGT
CGAATTTCTAATTATGGATGATGCGCTGCGCCGCGCGGTGATCAGCCGTACCGGCATAGGAGAGCTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACCACGATTGAAGAGGTCTTG
CGTGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)

Type II/IV secretion system protein (T2SSE)

(189-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.794

97.88

0.399
  pilB Vibrio campbellii strain DS40M4

40.7

95.936

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

40.441

96.113

0.389
  pilB Vibrio cholerae strain A1552

38.556

100

0.387
  pilB Legionella pneumophila strain ERS1305867

36.842

100

0.371

Multiple sequence alignment