Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Tchl_RS07630 Genome accession   NZ_CP018839
Coordinates   1645578..1647017 (-) Length   479 a.a.
NCBI ID   WP_075147870.1    Uniprot ID   A0A1H5WKU8
Organism   Thauera chlorobenzoica strain 3CB1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640578..1652017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tchl_RS07605 (Tchl_1526) - 1641383..1641994 (+) 612 WP_075147865.1 5-formyltetrahydrofolate cyclo-ligase -
  Tchl_RS07610 (Tchl_1527) ilvA 1642032..1643702 (-) 1671 WP_269745447.1 threonine ammonia-lyase, biosynthetic -
  Tchl_RS07615 (Tchl_1528) hprK 1643699..1644646 (-) 948 WP_075147867.1 HPr(Ser) kinase/phosphatase -
  Tchl_RS07620 (Tchl_1529) ptsN 1644615..1645100 (-) 486 WP_075147868.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Tchl_RS07625 (Tchl_1530) hpf 1645237..1645560 (-) 324 WP_075147869.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  Tchl_RS07630 (Tchl_1531) rpoN/rpoN1 1645578..1647017 (-) 1440 WP_075147870.1 RNA polymerase factor sigma-54 Machinery gene
  Tchl_RS07635 (Tchl_1532) lptB 1647040..1647765 (-) 726 WP_075147871.1 LPS export ABC transporter ATP-binding protein -
  Tchl_RS07640 (Tchl_1533) - 1647752..1648201 (-) 450 WP_075147872.1 regulatory protein RecX -
  Tchl_RS07645 (Tchl_1534) recA 1648218..1649258 (-) 1041 WP_075147873.1 recombinase RecA Machinery gene
  Tchl_RS07650 (Tchl_1535) - 1649518..1650006 (-) 489 WP_075147874.1 CinA family protein -
  Tchl_RS07655 (Tchl_1536) - 1650059..1650541 (-) 483 WP_075147875.1 phosphatidylglycerophosphatase A -
  Tchl_RS07660 (Tchl_1537) thiL 1650709..1651710 (-) 1002 WP_075147876.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53263.21 Da        Isoelectric Point: 5.0871

>NTDB_id=210886 Tchl_RS07630 WP_075147870.1 1645578..1647017(-) (rpoN/rpoN1) [Thauera chlorobenzoica strain 3CB1]
MKTSLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERFLLENPMLEREDGEDGEGGDGLPSSPGAAPVHDPSPAPGEG
EADTRAHDGTEDEGIDWSNVGQGAGSRGEDDDTDYQDLQAAGISLREHLDQQIALSPLSDRDRTLARLIIEALDDDGYLH
QPLEELLELLVPESGVELDDLAIALHHVQHLDPVGIAARTPQECLSLQLRTLPEDRTRTLALEIVDRHLALLAERNFAKL
KKLTGCDDDSLRAAQALICSLDPHPGMRYSLHETRYVLPDVIVRKLRGQWTVRLNQEAMPRLRINRLYASLLQQNRAQGG
GLGAQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVF
ELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDSKKPLSDARIAEVLGQQGIVVARRTIAKYRESLNIPPVSLRKSI

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=210886 Tchl_RS07630 WP_075147870.1 1645578..1647017(-) (rpoN/rpoN1) [Thauera chlorobenzoica strain 3CB1]
ATGAAAACCAGCCTCCAGCTCAAGCTTTCCCAGCACCTCACGCTGACCCCGCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCGACGATCGAGCTGAACCAGGAAATCGAGCGTTTCCTGCTCGAGAACCCGATGCTCGAGCGCGAGGACGGCGAGG
ACGGCGAAGGCGGGGATGGCCTCCCGTCCTCGCCCGGCGCCGCACCCGTGCACGACCCCTCGCCCGCCCCCGGCGAGGGC
GAGGCCGACACCCGCGCGCACGACGGCACCGAGGACGAAGGCATCGACTGGTCGAACGTCGGCCAGGGCGCCGGCAGCCG
CGGCGAAGACGACGACACCGACTACCAGGATCTTCAGGCCGCCGGCATCTCGCTGCGCGAGCACCTCGACCAGCAGATCG
CCCTGTCCCCGCTGTCGGACCGCGACCGCACGCTGGCGCGGCTGATCATCGAGGCCCTGGACGACGACGGCTACCTGCAT
CAGCCGCTGGAAGAACTCCTCGAGCTGCTCGTCCCCGAGTCCGGCGTCGAGCTCGACGACCTGGCGATCGCGTTGCACCA
CGTGCAGCACCTCGATCCGGTCGGCATCGCCGCGCGCACACCGCAGGAATGCCTGTCGCTGCAGCTGCGCACCCTGCCCG
AGGACCGCACCCGGACGCTGGCACTGGAGATCGTCGACAGGCACCTCGCGCTGCTCGCCGAGCGCAATTTCGCCAAGCTG
AAGAAGCTCACCGGCTGCGACGACGACAGCCTGCGCGCCGCCCAGGCGCTGATCTGCAGCCTCGACCCGCATCCGGGCAT
GCGCTACAGCCTGCACGAAACCCGCTACGTGCTGCCCGACGTCATCGTGCGCAAGCTGCGCGGGCAGTGGACGGTACGCC
TCAACCAGGAAGCCATGCCGCGCTTGCGGATCAACCGCCTGTACGCCAGCCTGCTGCAACAGAACCGCGCCCAGGGCGGC
GGACTCGGTGCCCAGTTGCAGGAAGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGCTTCGACACCATCCTGCGCGTTTC
CCAGGCGATCGTTGACCAGCAGCGGCAGTTCTTCGATCATGGAGACGTGGCGATGCGGCCACTCACTCTCCGGGAGATCG
CAGACCAGCTCGAGCTGCACGAATCCACCGTATCGCGCGTCACCACGCAGAAATTCATGGCGACCCCGCGCGGCGTATTC
GAACTCAAGTATTTCTTCGGCAGCCACGTCGCCACCGACACCGGTGGAGCGGCATCCTCGACGGCGATTCGTGCGCTGAT
TCGCCAGTTGGTCGATGCCGAAGACAGCAAGAAACCGCTATCCGACGCCAGGATTGCAGAAGTTCTGGGCCAGCAGGGTA
TCGTGGTCGCGCGGCGGACGATCGCCAAGTACCGCGAATCACTGAATATTCCGCCGGTCAGTCTGCGCAAGTCCATCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(115-308)

Sigma-54, DNA binding domain (Sigma54_DBD)

(324-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.8

100

0.582

Multiple sequence alignment