Detailed information NTG0021088

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LPG_RS04600
• Genome accession: NC_002942
• Coordinates: 1002679..1003743 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila subsp. pneumophila str. Philadelphia
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 997679..1008743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPG_RS04590 (lpg0925)	-	998716..1001100 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
LPG_RS04595 (lpg0926)	ravI	1001252..1002262 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
LPG_RS04600 (lpg0927)	pilM	1002679..1003743 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
LPG_RS04605 (lpg0928)	pilN	1003747..1004295 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
LPG_RS04610 (lpg0929)	pilO	1004309..1004908 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
LPG_RS04615 (lpg0930)	pilP	1004905..1005492 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
LPG_RS04620 (lpg0931)	pilQ	1005496..1007592 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
LPG_RS04625 (lpg0932)	aroK	1008044..1008565 (+)	522	WP_027219629.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=21088 LPG_RS04600 WP_010946662.1 1002679..1003743(+) (pilM) [Legionella pneumophila subsp. pneumophila str. Philadelphia]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=21088 LPG_RS04600 WP_010946662.1 1002679..1003743(+) (pilM) [Legionella pneumophila subsp. pneumophila str. Philadelphia]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418