Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   BSU_13700 Genome accession   NC_000964
Coordinates   1435628..1437727 (-) Length   699 a.a.
NCBI ID   NP_389253.1    Uniprot ID   O31673
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1430628..1442727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_13640 (BSU13640) spo0E 1430684..1430941 (+) 258 NP_389247.1 negative regulatory phosphatase acting on Spo0A-P (sporulation) -
  BSU_13650 (BSU13650) eag 1431027..1431458 (+) 432 NP_389248.1 putative small membrane protein -
  BSU_13660 (BSU13660) kinD 1431486..1433006 (-) 1521 NP_389249.1 histidine kinase phosphorylating Spo0A -
  BSU_13670 (BSU13670) mhqR 1433199..1433636 (+) 438 NP_389250.1 transcriptional regulator (MarR family) -
  BSU_13680 (BSU13680) motB 1433676..1434461 (-) 786 NP_389251.1 motility protein B; MotB component of the H+-coupled stator flagellum complex -
  BSU_13690 (BSU13690) motA 1434433..1435245 (-) 813 NP_389252.1 motility protein A; MotA component of the H+-coupled stator flagellum complex -
  BSU_13700 (BSU13700) clpE 1435628..1437727 (-) 2100 NP_389253.1 ATP-dependent Clp protease (class III stress gene) Regulator
  BSU_13710 (BSU13710) ykvI 1438092..1439135 (+) 1044 NP_389254.1 putative transporter -
  BSU_13720 (BSU13720) queC 1439448..1440107 (+) 660 NP_389255.1 pre-queuosine 0 synthase -
  BSU_13730 (BSU13730) queD 1440100..1440549 (+) 450 NP_389256.1 6-carboxy-5,6,7,8-tetrahydropterin synthase; queuosine biosynthesis -
  BSU_13740 (BSU13740) queE 1440542..1441273 (+) 732 NP_389257.1 7-carboxy-7-deazaguanine synthase -
  BSU_13750 (BSU13750) queF 1441291..1441788 (+) 498 NP_389258.1 NADPH-dependent 7-cyano-7-deazaguanine reductase -
  BSU_13760 (BSU13760) ykvN 1442347..1442703 (-) 357 NP_389259.1 putative transcriptional regulator (HGT island) -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77905.96 Da        Isoelectric Point: 5.0886

>NTDB_id=20979 BSU_13700 NP_389253.1 1435628..1437727(-) (clpE) [Bacillus subtilis subsp. subtilis str. 168]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=20979 BSU_13700 NP_389253.1 1435628..1437727(-) (clpE) [Bacillus subtilis subsp. subtilis str. 168]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACAGAGCGCAGCAAAACAAAGCGAAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTAAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCTCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCATGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATCGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAAGCGGTAAGACG
AAGCCGCGCCGGATTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAGGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCAAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA lid domain (AAA_lid_9)

(253-352)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O31673

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.847

89.7

0.429
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus pneumoniae D39

46.25

91.559

0.423
  clpC Streptococcus pneumoniae Rx1

46.25

91.559

0.423
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.85

88.555

0.415
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365

Multiple sequence alignment