Detailed information NTG0209633

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BG259_RS09645
• Genome accession: NZ_CP018680
• Coordinates: 2041358..2042227 (+)
• Length: 289 a.a.
• NCBI ID: WP_005446255.1
• Uniprot ID: A0A3A1Q6Q3
• Organism: Vibrio harveyi strain QT520
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2036358..2047227

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BG259_RS09620 (BG259_09620)	nadC	2036727..2037614 (+)	888	WP_074050812.1	carboxylating nicotinate-nucleotide diphosphorylase	-
BG259_RS09630 (BG259_09630)	pilA	2037879..2038337 (+)	459	WP_017189133.1	pilin	Machinery gene
BG259_RS09635 (BG259_09635)	pilB	2038343..2040028 (+)	1686	WP_074050813.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BG259_RS09640 (BG259_09640)	pilC	2040062..2041285 (+)	1224	WP_005446256.1	type II secretion system F family protein	Machinery gene
BG259_RS09645 (BG259_09645)	pilD	2041358..2042227 (+)	870	WP_005446255.1	prepilin peptidase	Machinery gene
BG259_RS09650 (BG259_09650)	coaE	2042228..2042842 (+)	615	WP_005446253.1	dephospho-CoA kinase	-
BG259_RS09655 (BG259_09655)	zapD	2042870..2043610 (+)	741	WP_050934119.1	cell division protein ZapD	-
BG259_RS09660 (BG259_09660)	yacG	2043673..2043867 (+)	195	WP_005446249.1	DNA gyrase inhibitor YacG	-
BG259_RS09665 (BG259_09665)	rplS	2044156..2044509 (-)	354	WP_005379971.1	50S ribosomal protein L19	-
BG259_RS09670 (BG259_09670)	trmD	2044551..2045294 (-)	744	WP_005446247.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
BG259_RS09675 (BG259_09675)	rimM	2045322..2045870 (-)	549	WP_005446245.1	ribosome maturation factor RimM	-
BG259_RS09680 (BG259_09680)	rpsP	2045899..2046147 (-)	249	WP_004410028.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32536.74 Da
• Isoelectric Point: 7.5422

>NTDB_id=209633 BG259_RS09645 WP_005446255.1 2041358..2042227(+) (pilD) [Vibrio harveyi strain QT520]
MEVFQYYPWLFVVFTAIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKETLTLSVPRSSCQQCGTHIRIR
DNIPVISWLLLKGKCHNCQSPISARYPLIELLTAFCSGFIAFHFGFSYFTVALVFFTFVLIAATFIDLDTMLLPDQLTLP
LMWAGIALALAGISPVSLQDSIIGAMAGYLCLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVSLIWGNQILNWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=209633 BG259_RS09645 WP_005446255.1 2041358..2042227(+) (pilD) [Vibrio harveyi strain QT520]
ATGGAAGTATTCCAGTACTACCCATGGCTATTCGTTGTATTTACCGCTATTTTTGGTTTGATCGTGGGTAGTTTCCTCAA
CGTAGTTATTTACCGTTTACCTAAGATCATGGAACTAGAATGGCGACGCGAGTGTGCTGAATCATTCCCTGAATACAAGA
TCGAGCCACCAAAAGAGACATTAACCTTAAGCGTGCCACGCTCATCTTGTCAGCAATGCGGTACACATATCCGCATTAGA
GACAATATCCCTGTGATTAGTTGGCTATTACTAAAAGGTAAATGCCACAACTGCCAATCACCTATCAGTGCCCGTTACCC
TCTTATTGAACTGCTTACCGCTTTTTGTTCGGGTTTTATCGCTTTTCATTTCGGTTTTAGCTACTTCACTGTGGCGCTGG
TTTTCTTTACCTTTGTATTGATTGCTGCCACATTTATCGACCTTGATACCATGTTGCTGCCAGACCAACTCACACTGCCA
TTGATGTGGGCGGGTATTGCTCTTGCTCTTGCCGGTATCAGCCCTGTGAGTCTTCAAGATTCTATTATTGGAGCAATGGC
AGGCTACTTATGTCTTTGGAGTGTTTATTGGTTATTCAAACTCCTCACAGGCAAAGAGGGAATGGGCTATGGTGACTTTA
AACTCCTTGCTGCATTAGGAGCTTGGCTGGGCTGGCAATCTCTGCCGATGATCATTTTACTTTCTTCGGTAGTAGGCGTC
ATTTTTGGTCTTATCCAGCTGCGCTTGCAAAAGCAAGGTATCGAAAAAGCCTTTCCTTTTGGTCCTTACCTTGCAATTGC
AGGATGGGTGAGCCTAATTTGGGGCAATCAAATCCTCAACTGGTACTTTACGTCGATTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A3A1Q6Q3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	97.232	100	0.972
pilD	Vibrio cholerae strain A1552	74.306	99.654	0.74
pilD	Acinetobacter baumannii D1279779	52.768	93.772	0.495
pilD	Acinetobacter nosocomialis M2	52.399	93.772	0.491
pilD	Neisseria gonorrhoeae MS11	45.652	95.502	0.436