Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BS064_RS01810 Genome accession   NZ_CP018254
Coordinates   380366..381106 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain AF-401     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375366..386106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS064_RS01795 (BS064_01785) ponA 375953..378508 (-) 2556 WP_000736683.1 penicillin-binding protein PBP1a -
  BS064_RS01800 (BS064_01790) pilM 378670..379728 (+) 1059 WP_073392535.1 pilus assembly protein PilM Machinery gene
  BS064_RS01805 (BS064_01795) pilN 379728..380369 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  BS064_RS01810 (BS064_01800) pilO 380366..381106 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  BS064_RS01815 (BS064_01805) pilP 381117..381644 (+) 528 WP_000695058.1 pilus assembly protein PilP Machinery gene
  BS064_RS01820 (BS064_01810) pilQ 381707..383872 (+) 2166 WP_001017038.1 type IV pilus secretin PilQ family protein Machinery gene
  BS064_RS01825 (BS064_01815) aroK 383884..384453 (+) 570 WP_000643712.1 shikimate kinase AroK -
  BS064_RS01830 (BS064_01820) aroB 384482..385564 (+) 1083 WP_001194002.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=207670 BS064_RS01810 WP_000076101.1 380366..381106(+) (pilO) [Acinetobacter baumannii strain AF-401]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=207670 BS064_RS01810 WP_000076101.1 380366..381106(+) (pilO) [Acinetobacter baumannii strain AF-401]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment