Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BSF20_RS14315 Genome accession   NZ_CP018152
Coordinates   2746359..2747126 (+) Length   255 a.a.
NCBI ID   WP_003152982.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2741359..2752126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS14285 (BSF20_13995) - 2741642..2743774 (+) 2133 WP_015387990.1 HD family phosphohydrolase -
  BSF20_RS14290 (BSF20_14000) ybeY 2743775..2744248 (+) 474 WP_015387991.1 rRNA maturation RNase YbeY -
  BSF20_RS14295 (BSF20_14005) - 2744229..2744603 (+) 375 WP_003152974.1 diacylglycerol kinase family protein -
  BSF20_RS14300 (BSF20_14010) - 2744727..2745137 (+) 411 WP_003152976.1 cytidine deaminase -
  BSF20_RS14305 (BSF20_14015) era 2745118..2746023 (+) 906 WP_003152978.1 GTPase Era -
  BSF20_RS14310 (BSF20_14020) - 2746179..2746322 (+) 144 WP_007408286.1 YqzL family protein -
  BSF20_RS14315 (BSF20_14025) recO 2746359..2747126 (+) 768 WP_003152982.1 DNA repair protein RecO Machinery gene
  BSF20_RS14320 (BSF20_14030) glyQ 2747428..2748315 (+) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  BSF20_RS14325 (BSF20_14035) glyS 2748308..2750347 (+) 2040 WP_003152986.1 glycine--tRNA ligase subunit beta -
  BSF20_RS14330 (BSF20_14040) ccpN 2750448..2751086 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  BSF20_RS14335 (BSF20_14045) - 2751112..2751924 (+) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29264.86 Da        Isoelectric Point: 8.3099

>NTDB_id=206544 BSF20_RS14315 WP_003152982.1 2746359..2747126(+) (recO) [Bacillus amyloliquefaciens strain LM2303]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKQVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=206544 BSF20_RS14315 WP_003152982.1 2746359..2747126(+) (recO) [Bacillus amyloliquefaciens strain LM2303]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACAGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.412

100

0.894

Multiple sequence alignment