Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A2U21_RS09225 Genome accession   NZ_CP018034
Coordinates   1801759..1802340 (+) Length   193 a.a.
NCBI ID   WP_021110392.1    Uniprot ID   A0A084ELH5
Organism   Glaesserella parasuis str. Nagasaki     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1796759..1807340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2U21_RS09185 (A2U21_08885) gltX 1797375..1798814 (-) 1440 WP_021110394.1 glutamate--tRNA ligase -
  A2U21_RS09210 (A2U21_08910) - 1799648..1799986 (+) 339 WP_021110393.1 selenocysteine synthase -
  A2U21_RS09215 (A2U21_08915) - 1799990..1800298 (+) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  A2U21_RS09220 (A2U21_08920) - 1800499..1801659 (+) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  A2U21_RS09225 (A2U21_08925) pilF 1801759..1802340 (+) 582 WP_021110392.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A2U21_RS09230 (A2U21_08930) - 1802401..1803336 (+) 936 WP_021110391.1 KpsF/GutQ family sugar-phosphate isomerase -
  A2U21_RS09235 (A2U21_08935) greB 1803336..1803815 (+) 480 WP_021110390.1 transcription elongation factor GreB -
  A2U21_RS09255 (A2U21_08955) - 1804349..1805647 (+) 1299 WP_021110389.1 NAD(P)/FAD-dependent oxidoreductase -
  A2U21_RS09260 (A2U21_08960) - 1805732..1806265 (-) 534 WP_015940082.1 TIGR00645 family protein -
  A2U21_RS09265 (A2U21_08965) - 1806354..1806992 (-) 639 WP_016528334.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.24 Da        Isoelectric Point: 8.0872

>NTDB_id=205216 A2U21_RS09225 WP_021110392.1 1801759..1802340(+) (pilF) [Glaesserella parasuis str. Nagasaki]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=205216 A2U21_RS09225 WP_021110392.1 1801759..1802340(+) (pilF) [Glaesserella parasuis str. Nagasaki]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A084ELH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

99.482

100

0.995
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415

Multiple sequence alignment