Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A2U20_RS02730 Genome accession   NZ_CP018032
Coordinates   514539..515120 (-) Length   193 a.a.
NCBI ID   WP_021110392.1    Uniprot ID   A0A084ELH5
Organism   Glaesserella parasuis D74     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509539..520120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2U20_RS02690 (A2U20_02655) - 509887..510525 (+) 639 WP_005710397.1 nitroreductase family protein -
  A2U20_RS02695 (A2U20_02660) - 510614..511147 (+) 534 WP_010786060.1 TIGR00645 family protein -
  A2U20_RS02700 (A2U20_02665) - 511232..512530 (-) 1299 WP_021112015.1 NAD(P)/FAD-dependent oxidoreductase -
  A2U20_RS02720 (A2U20_02685) greB 513064..513543 (-) 480 WP_021117804.1 transcription elongation factor GreB -
  A2U20_RS02725 (A2U20_02690) - 513543..514478 (-) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  A2U20_RS02730 (A2U20_02695) pilF 514539..515120 (-) 582 WP_021110392.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A2U20_RS02735 (A2U20_02700) - 515272..516432 (-) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  A2U20_RS12105 (A2U20_02705) - 516633..516941 (-) 309 WP_021117805.1 helix-turn-helix domain-containing protein -
  A2U20_RS12110 (A2U20_02710) - 516945..517283 (-) 339 WP_021117806.1 type II toxin-antitoxin system RelE/ParE family toxin -
  A2U20_RS02770 (A2U20_02735) gltX 518119..519558 (+) 1440 WP_043895984.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.24 Da        Isoelectric Point: 8.0872

>NTDB_id=205173 A2U20_RS02730 WP_021110392.1 514539..515120(-) (pilF) [Glaesserella parasuis D74]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=205173 A2U20_RS02730 WP_021110392.1 514539..515120(-) (pilF) [Glaesserella parasuis D74]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAAAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTATAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A084ELH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

99.482

100

0.995
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415

Multiple sequence alignment