Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   BMF22_RS05535 Genome accession   NZ_CP017961
Coordinates   1129851..1130396 (+) Length   181 a.a.
NCBI ID   WP_005752449.1    Uniprot ID   -
Organism   Pasteurella multocida strain Razi_Pm0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1124851..1135396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMF22_RS05510 (BMF22_05495) - 1125276..1126205 (+) 930 WP_005725108.1 phosphatidate cytidylyltransferase -
  BMF22_RS05515 (BMF22_05500) mog 1126231..1126809 (+) 579 WP_005737641.1 molybdopterin adenylyltransferase -
  BMF22_RS05520 (BMF22_05505) glnB 1126854..1127192 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  BMF22_RS05525 (BMF22_05510) - 1127193..1128446 (+) 1254 WP_005752439.1 AI-2E family transporter -
  BMF22_RS05530 (BMF22_05515) - 1128627..1129811 (+) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  BMF22_RS05535 (BMF22_05520) pilF/pilF2 1129851..1130396 (+) 546 WP_005752449.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BMF22_RS05540 (BMF22_05525) - 1130519..1131475 (+) 957 WP_005725132.1 RodZ domain-containing protein -
  BMF22_RS05545 (BMF22_05530) ispG 1131502..1132605 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BMF22_RS05550 (BMF22_05535) hisS 1132635..1133906 (+) 1272 WP_005752452.1 histidine--tRNA ligase -
  BMF22_RS05555 (BMF22_05540) - 1133925..1134536 (+) 612 WP_005725137.1 YfgM family protein -
  BMF22_RS05560 (BMF22_05545) - 1134748..1135185 (+) 438 WP_005725139.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20911.47 Da        Isoelectric Point: 7.8913

>NTDB_id=204481 BMF22_RS05535 WP_005752449.1 1129851..1130396(+) (pilF/pilF2) [Pasteurella multocida strain Razi_Pm0001]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=204481 BMF22_RS05535 WP_005752449.1 1129851..1130396(+) (pilF/pilF2) [Pasteurella multocida strain Razi_Pm0001]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCTAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50.568

97.238

0.492
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459

Multiple sequence alignment