Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   K04M5_RS14700 Genome accession   NZ_CP017899
Coordinates   3106876..3107451 (-) Length   191 a.a.
NCBI ID   WP_017821732.1    Uniprot ID   A0A2I3CBW9
Organism   Vibrio alginolyticus strain K04M5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101876..3112451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K04M5_RS14675 (K04M5_28730) aroB 3102175..3103275 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  K04M5_RS14680 (K04M5_28740) aroK 3103308..3103826 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K04M5_RS14685 (K04M5_28750) pilQ 3104014..3105759 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  K04M5_RS14690 (K04M5_28760) pilP 3105785..3106300 (-) 516 WP_086047068.1 pilus assembly protein PilP Machinery gene
  K04M5_RS14695 (K04M5_28770) pilO 3106290..3106883 (-) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  K04M5_RS14700 (K04M5_28780) pilN 3106876..3107451 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K04M5_RS14705 (K04M5_28790) pilM 3107435..3108451 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  K04M5_RS14710 (K04M5_28800) - 3108596..3111157 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  K04M5_RS14715 (K04M5_28810) oxyR 3111211..3112122 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22349.86 Da        Isoelectric Point: 7.4658

>NTDB_id=203823 K04M5_RS14700 WP_017821732.1 3106876..3107451(-) (pilN) [Vibrio alginolyticus strain K04M5]
MLYRINLLPWRESQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQERLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=203823 K04M5_RS14700 WP_017821732.1 3106876..3107451(-) (pilN) [Vibrio alginolyticus strain K04M5]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAGTCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGAGC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGTCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCTGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3CBW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

63.277

92.67

0.586

Multiple sequence alignment