Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K04M5_RS14705 Genome accession   NZ_CP017899
Coordinates   3107435..3108451 (-) Length   338 a.a.
NCBI ID   WP_017821733.1    Uniprot ID   A0AAW7A029
Organism   Vibrio alginolyticus strain K04M5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3102435..3113451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K04M5_RS14680 (K04M5_28740) aroK 3103308..3103826 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K04M5_RS14685 (K04M5_28750) pilQ 3104014..3105759 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  K04M5_RS14690 (K04M5_28760) pilP 3105785..3106300 (-) 516 WP_086047068.1 pilus assembly protein PilP Machinery gene
  K04M5_RS14695 (K04M5_28770) pilO 3106290..3106883 (-) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  K04M5_RS14700 (K04M5_28780) pilN 3106876..3107451 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K04M5_RS14705 (K04M5_28790) pilM 3107435..3108451 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  K04M5_RS14710 (K04M5_28800) - 3108596..3111157 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  K04M5_RS14715 (K04M5_28810) oxyR 3111211..3112122 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  K04M5_RS14720 (K04M5_28820) - 3112292..3113020 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37595.95 Da        Isoelectric Point: 7.2224

>NTDB_id=203824 K04M5_RS14705 WP_017821733.1 3107435..3108451(-) (pilM) [Vibrio alginolyticus strain K04M5]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIIERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=203824 K04M5_RS14705 WP_017821733.1 3107435..3108451(-) (pilM) [Vibrio alginolyticus strain K04M5]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCATAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAGGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5

Multiple sequence alignment