Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K04M3_RS13430 Genome accession   NZ_CP017896
Coordinates   2856940..2857404 (+) Length   154 a.a.
NCBI ID   WP_033905592.1    Uniprot ID   A0AAW9B0I7
Organism   Vibrio alginolyticus isolate K04M3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2851940..2862404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K04M3_RS13415 (K04M3_26240) pdhR 2853973..2854740 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  K04M3_RS13420 (K04M3_26250) ampD 2855146..2855697 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K04M3_RS13425 (K04M3_26260) nadC 2855790..2856677 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K04M3_RS13430 (K04M3_26270) pilA 2856940..2857404 (+) 465 WP_033905592.1 pilin Machinery gene
  K04M3_RS13435 (K04M3_26280) pilB 2857408..2859093 (+) 1686 WP_054730502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K04M3_RS13440 (K04M3_26290) pilC 2859117..2860340 (+) 1224 WP_054730505.1 type II secretion system F family protein Machinery gene
  K04M3_RS13445 (K04M3_26300) pilD 2860405..2861274 (+) 870 WP_086047031.1 prepilin peptidase Machinery gene
  K04M3_RS13450 (K04M3_26310) coaE 2861275..2861889 (+) 615 WP_017635920.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16098.26 Da        Isoelectric Point: 7.6284

>NTDB_id=203758 K04M3_RS13430 WP_033905592.1 2856940..2857404(+) (pilA) [Vibrio alginolyticus isolate K04M3]
MKNSKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAASAMKLAVELCAHENASNAATFKTNCLSGS
NGVPSKINNLNDISIEAVAGSSGVNVIAKAVAVKGPIASGEQYTMASNYTTSGIEWAGSCQDSNGNNQSDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=203758 K04M3_RS13430 WP_033905592.1 2856940..2857404(+) (pilA) [Vibrio alginolyticus isolate K04M3]
ATGAAAAACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTAATGATTGTCGTCGCGATTATTGGTATTTT
GGCCGCATTTGCTGTGCCTGCTTATCAAAACTACACAAAAAAAGCGACGCTGGCAGAGTTCCCAAAAGCAGCTTCTGCGA
TGAAGTTAGCCGTTGAGTTGTGTGCACATGAAAATGCATCAAACGCAGCTACTTTTAAAACGAACTGCTTATCTGGTAGT
AATGGTGTACCTTCAAAAATTAACAACCTAAATGATATCTCTATTGAAGCAGTTGCTGGATCATCAGGCGTAAATGTCAT
TGCAAAAGCTGTAGCAGTAAAAGGGCCGATTGCTAGTGGAGAACAGTATACTATGGCATCTAACTACACGACTAGTGGTA
TTGAGTGGGCGGGCTCATGTCAAGATTCTAATGGTAATAACCAATCTGATTACTGTCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

55.484

100

0.558

Multiple sequence alignment